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Literature summary for 2.3.1.43 extracted from
- Characterization of lecithin:cholesterol acyltransferase expressed in a human lung cell line (2004), Protein Expr. Purif., 36, 157-164.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| apolipoprotein A-I | - |
Homo sapiens |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the recombinant enzyme is utilized for enzyme replacement therapy in case of congenital enzyme deficiency | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| optimization of expression in human lung cell line H1299, enzyme is secreted to the culture medium | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00056 | - |
1,2-bis[4-(1-pyreno)butanoyl]-sn-glycero-3-phosphocholine | fluorescent assay, pH 7.4, 25°C, recombinant enzyme | Homo sapiens |  |
| 0.0006 | - |
phosphatidylcholine | radioassay, pH 7.4, 37°C, recombinant enzyme | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Homo sapiens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
x * 64000, recombinant glycosylated enzyme, SDS-PAGE, x * 44000, recombinant deglycosylated enzyme, SDS-PAGE | Homo sapiens |
| 64000 | - |
x * 64000, recombinant glycosylated enzyme, SDS-PAGE, x * 44000, recombinant deglycosylated enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | enzyme deficiency can cause corneal opacity, proteinuria, anemia, and kidney failure | ? | - |
? | |
| phosphatidylcholine + cholesterol | Homo sapiens | - |
1-acylglycerophosphocholine + cholesteryl ester | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P04180 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | analysis of sugar composition, overview | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from human lung cell line H1299 culture medium | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
| plasma | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activities produced by different lung cell clones expressing the enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-bis[4-(1-pyreno)butanoyl]-sn-glycero-3-phosphocholine + cholesterol | fluorescent substrate assay | Homo sapiens | ? | - |
? | |
| additional information | enzyme deficiency can cause corneal opacity, proteinuria, anemia, and kidney failure | Homo sapiens | ? | - |
? | |
| phosphatidylcholine + cholesterol | - |
Homo sapiens | 1-acylglycerophosphocholine + cholesteryl ester | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 64000, recombinant glycosylated enzyme, SDS-PAGE, x * 44000, recombinant deglycosylated enzyme, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LCAT | - |
Homo sapiens |
| lecithin:cholesterol acyltransferase | - |
Homo sapiens |
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