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Literature summary for 2.3.1.43 extracted from

  • Pszenny, V.; Ehrenman, K.; Romano, J.D.; Kennard, A.; Schultz, A.; Roos, D.S.; Grigg, M.E.; Carruthers, V.B.; Coppens, I.
    A lipolytic lecithin:cholesterol acyltransferase secreted by Toxoplasma facilitates parasite replication and egress (2015), J. Biol. Chem., 291, 3725-3746.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.43

Cloned(Commentary)

Cloned (Comment) Organism
gene lcat, located on chromosome VIII, DNA and amino acid sequence determination and analyis, sequence comparisons and phylogenetic analysis, recombinant stable expression of HA- or YFP-tagged enzyme in Toxoplasma gondii, recombinant expression of His-tagged peptide TgLCAT(106-202) in Escherichia coli strain M15, recombinant expression in insect cells, using baculovirus transfection, and secretion to the culture medium Toxoplasma gondii

Protein Variants

Protein Variants Comment Organism
additional information construction of enzyme knockout and overexpressing strains, the loss of TgLCAT expression leads to an egress defect, and its overexpression confers an egress advantage, phenotype, overview Toxoplasma gondii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000026
-
 phosphatidylcholine purified recombinant enzyme, pH 7.5, 37°C Toxoplasma gondii

Localization

Localization Comment Organism GeneOntology No. Textmining
dense core granule a secretory particle of the parasite, detected within the host cell in the lumen of the parasitophorous vacuole. The enzyme localizes in the matrix of the organelles or at the limiting membrane of dense granules. Localization of TgLCAT to the surface of axenic parasites Toxoplasma gondii 31045
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no effect by EDTA and excess calcium on enzyme activity Toxoplasma gondii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
92000
-
 x * 92000, recombinant enzyme, SDS-PAGE Toxoplasma gondii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Toxoplasma gondii the enzyme from Toxoplasma gondii also shows phospholipase and hemolytic activity, recombinant TgLCAT has a dual enzymatic activity as a calcium-independent phospholipase A2 and a cholesterol transacyltransferase ?
-
 ?
additional information Toxoplasma gondii ME49 the enzyme from Toxoplasma gondii also shows phospholipase and hemolytic activity, recombinant TgLCAT has a dual enzymatic activity as a calcium-independent phospholipase A2 and a cholesterol transacyltransferase ?
-
 ?
phosphatidylcholine + a sterol Toxoplasma gondii
-
 1-acylglycerophosphocholine + a sterol ester
-
 ?
phosphatidylcholine + a sterol Toxoplasma gondii ME49
-
 1-acylglycerophosphocholine + a sterol ester
-
 ?

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii S8GJB1 gene lcat
-
Toxoplasma gondii ME49 S8GJB1 gene lcat
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Spodoptera frugiperda Sf21 cells by immunoaffinity chromatography, recombinant His-tagged peptide TgLCAT(106-202) from Escherichia coli strain M15 by nickel affinity chromatography Toxoplasma gondii

Source Tissue

Source Tissue Comment Organism Textmining
additional information the protozoan parasite Toxoplasma gondii develops within a parasitophorous vacuole in mammalian cells Toxoplasma gondii
-
tachyzoite
-
 Toxoplasma gondii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00078
-
 purified recombinant enzyme, pH 7.5, 37°C Toxoplasma gondii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme from Toxoplasma gondii also shows phospholipase and hemolytic activity, recombinant TgLCAT has a dual enzymatic activity as a calcium-independent phospholipase A2 and a cholesterol transacyltransferase Toxoplasma gondii ?
-
 ?
additional information the enzyme from Toxoplasma gondii also shows phospholipase and hemolytic activity, recombinant TgLCAT has a dual enzymatic activity as a calcium-independent phospholipase A2 and a cholesterol transacyltransferase Toxoplasma gondii ME49 ?
-
 ?
phosphatidylcholine + a sterol
-
 Toxoplasma gondii 1-acylglycerophosphocholine + a sterol ester
-
 ?
phosphatidylcholine + a sterol
-
 Toxoplasma gondii ME49 1-acylglycerophosphocholine + a sterol ester
-
 ?

Subunits

Subunits Comment Organism
? x * 92000, recombinant enzyme, SDS-PAGE Toxoplasma gondii

Synonyms

Synonyms Comment Organism
cholesterol transacyltransferase
-
 Toxoplasma gondii
TgLCAT
-
 Toxoplasma gondii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Toxoplasma gondii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Toxoplasma gondii

General Information

General Information Comment Organism
malfunction Toxoplasma gondii lacking LCAT shows delayed egress whereas parasites overexpressing LCAT exit faster from host cells than parental parasites Toxoplasma gondii
physiological function the protozoan parasite Toxoplasma gondii develops within a parasitophorous vacuole in mammalian cells, where it scavenges cholesterol. When cholesterol is present in excess in its environment, the parasite expulses this lipid into the parasitophorous vacuole or esterifies it for storage in lipid bodies. The unique enzyme from Toxoplasma gondii is a homologue of mammalian lecithin:cholesterol acyltransferase (LCAT), a key enzyme that produces cholesteryl esters via transfer of acyl groups from phospholipids to the 3-OH of free cholesterol, leading to the removal of excess cholesterol from tissues Toxoplasma gondii