Cloned (Comment) | Organism |
---|---|
overexpression of metA gene in Escherichia coli BL21 (DE3) | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | inhibition is pH-dependent | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
succinyl-CoA | recombinant protein | Escherichia coli | |
0.18 | - |
glutaryl-CoA | recombinant protein | Escherichia coli | |
0.64 | - |
coenzyme A | recombinant protein | Escherichia coli | |
1.6 | - |
L-homoserine | recombinant protein | Escherichia coli | |
3.5 | - |
O-succinylhomoserine | recombinant protein | Escherichia coli | |
10 | - |
D-homoserine | recombinant protein | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35600 | - |
2 * 35600, SDS-PAGE | Escherichia coli |
86000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + L-homoserine | Escherichia coli | involved in cystathionine and methionine biosynthesis | CoA + O-succinyl-L-homoserine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
metA gene | - |
Purification (Comment) | Organism |
---|---|
recombinant from E. coli BL21 (DE3) | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
succinyl-CoA + L-homoserine = CoA + O-succinyl-L-homoserine | ping-pong mechanism, enzyme contains a cysteine in the active site, member of the acyltransferase family | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutaryl-CoA + L-homoserine | - |
Escherichia coli | CoA + O-glutaryl-L-homoserine | - |
r | |
additional information | 3-amino-1-propanol, L-serine, and L-threonine are poor substrates | Escherichia coli | ? | - |
? | |
additional information | no activity with acetyl-CoA, propionyl-CoA, butyryl-CoA, crotonyl-CoA, malonyl-CoA, and gamma-hydroxybutyric acid, and gamma-aminobutyric acid | Escherichia coli | ? | - |
? | |
succinyl-CoA + D-homoserine | - |
Escherichia coli | CoA + O-succinyl-D-homoserine | - |
r | |
succinyl-CoA + L-homoserine | - |
Escherichia coli | CoA + O-succinyl-L-homoserine | - |
r | |
succinyl-CoA + L-homoserine | involved in cystathionine and methionine biosynthesis | Escherichia coli | CoA + O-succinyl-L-homoserine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35600, SDS-PAGE | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
glutaryl-CoA | recombinant protein | Escherichia coli | |
5.23 | - |
coenzyme A | recombinant protein | Escherichia coli | |
5.23 | - |
O-succinyl-L-homoserine | recombinant protein | Escherichia coli | |
12 | - |
D-homoserine | recombinant protein | Escherichia coli | |
24 | - |
succinyl-CoA | recombinant protein | Escherichia coli | |
24 | - |
L-homoserine | recombinant protein | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
broad maximum | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pKs of 6.6 and about 7.9 | Escherichia coli |
6 | 8.7 | - |
Escherichia coli |