Cloned (Comment) | Organism |
---|---|
GST-fusion protein expressed in Escherichia coli JM109 | Escherichia coli |
GST-fusion protein expressed in Escherichia coli JM109 | Symbiobacterium thermophilum |
GST-fusion protein expressed in Escherichia coli JM109 | Acetivibrio thermocellus |
GST-fusion protein expressed in Escherichia coli JM109 | Aquifex aeolicus |
GST-fusion protein expressed in Escherichia coli JM109 | Geobacillus kaustophilus |
His-tagged protein expressed in Escherichia coli Rosetta-gami (DE3) | Thermosynechococcus vestitus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
pimeloyl-CoA | pH 6.0, 60°C | Aquifex aeolicus | |
0.02 | - |
pimeloyl-CoA | pH 6.0, 60°C | Geobacillus kaustophilus | |
0.025 | - |
pimeloyl-CoA | pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.03 | - |
pimeloyl-CoA | pH 6.0, 60°C | Symbiobacterium thermophilum | |
0.09 | - |
L-alanine | pH 6.0, 60°C | Geobacillus kaustophilus | |
0.23 | - |
L-alanine | pH 6.0, 60°C | Aquifex aeolicus | |
0.5 | - |
L-alanine | pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
2.28 | - |
L-alanine | pH 6.0, 60°C | Symbiobacterium thermophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
x * 44000, SDS-PAGE | Escherichia coli |
44000 | - |
x * 44000, SDS-PAGE | Geobacillus kaustophilus |
45000 | - |
x * 45000, SDS-PAGE | Acetivibrio thermocellus |
45000 | - |
x * 45000, SDS-PAGE | Aquifex aeolicus |
45000 | - |
x * 45000, SDS-PAGE | Thermosynechococcus vestitus |
46000 | - |
x * 46000, SDS-PAGE | Symbiobacterium thermophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
- |
- |
Acetivibrio thermocellus ATCC 27405 | - |
- |
- |
Aquifex aeolicus | - |
- |
- |
Escherichia coli | - |
- |
- |
Geobacillus kaustophilus | - |
- |
- |
Geobacillus kaustophilus JCM 12893 | - |
- |
- |
Symbiobacterium thermophilum | - |
- |
- |
Symbiobacterium thermophilum IAM14863 | - |
- |
- |
Thermosynechococcus vestitus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography | Acetivibrio thermocellus |
Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography, gel filtration | Aquifex aeolicus |
Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography, heat treatment with centrifugation | Symbiobacterium thermophilum |
Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography, ion exchange chromatography | Escherichia coli |
Glutathione Sepharose chromatography, GST-tag cleavage, Glutathione Sepharose chromatography, ion exchange chromatography | Geobacillus kaustophilus |
immobilized metal ion affinity chromatography (Ni2+), His-tag cleavage, immobilized metal ion affinity chromatography (Ni2+), gel filtration | Thermosynechococcus vestitus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.032 | - |
glycine, pimeloyl-CoA, pH 6.0, 60°C | Symbiobacterium thermophilum |
0.071 | - |
L-alanine, pimeloyl-CoA, pH 6.0, 60°C | Geobacillus kaustophilus |
0.078 | - |
L-alanine, pimeloyl-CoA, pH 6.0, 60°C | Symbiobacterium thermophilum |
0.272 | - |
L-alanine, acetyl-CoA, pH 6.0, 60°C | Symbiobacterium thermophilum |
0.897 | - |
glycine, acetyl-CoA, pH 6.0, 60°C | Symbiobacterium thermophilum |
2.46 | - |
L-alanine, pimeloyl-CoA, pH 6.0, 60°C | Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycine + acetyl-CoA | - |
Symbiobacterium thermophilum | ? | - |
? | |
glycine + acetyl-CoA | - |
Symbiobacterium thermophilum IAM14863 | ? | - |
? | |
glycine + pimeloyl-CoA | - |
Symbiobacterium thermophilum | ? | - |
? | |
glycine + pimeloyl-CoA | - |
Symbiobacterium thermophilum IAM14863 | ? | - |
? | |
L-alanine + acetyl-CoA | - |
Symbiobacterium thermophilum | ? | - |
? | |
L-alanine + acetyl-CoA | - |
Symbiobacterium thermophilum IAM14863 | ? | - |
? | |
L-alanine + pimeloyl-CoA | - |
Escherichia coli | 8-amino-7-oxononanoate + CoA + CO2 | - |
? | |
L-alanine + pimeloyl-CoA | - |
Acetivibrio thermocellus | 8-amino-7-oxononanoate + CoA + CO2 | - |
? | |
L-alanine + pimeloyl-CoA | - |
Thermosynechococcus vestitus | 8-amino-7-oxononanoate + CoA + CO2 | - |
? | |
L-alanine + pimeloyl-CoA | no activity observed with acetyl-CoA, no activity observed with glycine or L-serine | Aquifex aeolicus | 8-amino-7-oxononanoate + CoA + CO2 | - |
? | |
L-alanine + pimeloyl-CoA | no activity observed with acetyl-CoA, no activity observed with glycine or L-serine | Geobacillus kaustophilus | 8-amino-7-oxononanoate + CoA + CO2 | - |
? | |
L-alanine + pimeloyl-CoA | no activity observed with L-serine | Symbiobacterium thermophilum | 8-amino-7-oxononanoate + CoA + CO2 | - |
? | |
L-alanine + pimeloyl-CoA | no activity observed with acetyl-CoA, no activity observed with glycine or L-serine | Geobacillus kaustophilus JCM 12893 | 8-amino-7-oxononanoate + CoA + CO2 | - |
? | |
L-alanine + pimeloyl-CoA | no activity observed with L-serine | Symbiobacterium thermophilum IAM14863 | 8-amino-7-oxononanoate + CoA + CO2 | - |
? | |
L-alanine + pimeloyl-CoA | - |
Acetivibrio thermocellus ATCC 27405 | 8-amino-7-oxononanoate + CoA + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Acetivibrio thermocellus |
? | x * 45000, SDS-PAGE | Aquifex aeolicus |
? | x * 45000, SDS-PAGE | Thermosynechococcus vestitus |
? | x * 46000, SDS-PAGE | Symbiobacterium thermophilum |
? | x * 44000, SDS-PAGE | Escherichia coli |
? | x * 44000, SDS-PAGE | Geobacillus kaustophilus |
Synonyms | Comment | Organism |
---|---|---|
7-keto-8-aminopelargonic acid synthase | - |
Escherichia coli |
7-keto-8-aminopelargonic acid synthase | - |
Symbiobacterium thermophilum |
7-keto-8-aminopelargonic acid synthase | - |
Acetivibrio thermocellus |
7-keto-8-aminopelargonic acid synthase | - |
Aquifex aeolicus |
7-keto-8-aminopelargonic acid synthase | - |
Thermosynechococcus vestitus |
7-keto-8-aminopelargonic acid synthase | - |
Geobacillus kaustophilus |
8-amino-7-oxononanoate synthase | - |
Escherichia coli |
8-amino-7-oxononanoate synthase | - |
Symbiobacterium thermophilum |
8-amino-7-oxononanoate synthase | - |
Acetivibrio thermocellus |
8-amino-7-oxononanoate synthase | - |
Aquifex aeolicus |
8-amino-7-oxononanoate synthase | - |
Thermosynechococcus vestitus |
8-amino-7-oxononanoate synthase | - |
Geobacillus kaustophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Escherichia coli |
50 | 60 | - |
Symbiobacterium thermophilum |
50 | 60 | - |
Acetivibrio thermocellus |
50 | 60 | - |
Aquifex aeolicus |
50 | 60 | - |
Thermosynechococcus vestitus |
50 | 60 | - |
Geobacillus kaustophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable for one h | Escherichia coli |
55 | - |
stable for one h | Symbiobacterium thermophilum |
65 | - |
stable for one h | Thermosynechococcus vestitus |
70 | - |
stable for one h | Acetivibrio thermocellus |
75 | - |
stable for one h | Geobacillus kaustophilus |
93 | - |
stable for one h | Aquifex aeolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Escherichia coli | |
pyridoxal 5'-phosphate | - |
Symbiobacterium thermophilum | |
pyridoxal 5'-phosphate | - |
Acetivibrio thermocellus | |
pyridoxal 5'-phosphate | - |
Aquifex aeolicus | |
pyridoxal 5'-phosphate | - |
Thermosynechococcus vestitus | |
pyridoxal 5'-phosphate | - |
Geobacillus kaustophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
L-alanine | pH 6.0, 60°C | Symbiobacterium thermophilum | |
0.984 | - |
L-alanine | pH 6.0, 60°C | Geobacillus kaustophilus | |
1.5 | - |
pimeloyl-CoA | pH 6.0, 60°C | Symbiobacterium thermophilum | |
2.4 | - |
pimeloyl-CoA | pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
4.9 | - |
pimeloyl-CoA | pH 6.0, 60°C | Geobacillus kaustophilus | |
7.33 | - |
L-alanine | pH 6.0, 60°C | Aquifex aeolicus | |
213 | - |
pimeloyl-CoA | pH 6.0, 60°C | Aquifex aeolicus |