Literature summary for 2.3.1.47 extracted from

Fan, S.; Li, D.F.; Wang, D.C.; Fleming, J.; Zhang, H.; Zhou, Y.; Zhou, L.; Zhou, J.; Chen, T.; Chen, G.; Zhang, X.E.; Bi, L.
Structure and function of Mycobacterium smegmatis 7-keto-8-aminopelargonic acid (KAPA) synthase (2015), Int. J. Biochem. Cell Biol., 58, 71-80.

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Application

Application	Comment	Organism
drug development	the enzyme is a target for development of drugs against mycobac-terial pathogens	Mycolicibacterium smegmatis

Cloned(Commentary)

Cloned (Comment)	Organism
gene bioF, the enzyme coding gene is located between genes bioA(MSMEG 3188) and bioD (MSMEG 3190), recombinant His-tagged enzyme expression in Escherichia coli strain BL21(DE3)	Mycolicibacterium smegmatis

Crystallization (Commentary)

Crystallization (Comment)	Organism
the crystal structure of MsBioF is solved by molecular replacement using the structure of EcAONS, PDB code 1BS0, as the search model	Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
pimeloyl-[acyl-carrier protein] + L-alanine	Mycolicibacterium smegmatis	-	8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]	-	?
pimeloyl-[acyl-carrier protein] + L-alanine	Mycolicibacterium smegmatis ATCC 700084	-	8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycolicibacterium smegmatis	A0QX65	gene bioF	-
Mycolicibacterium smegmatis ATCC 700084	A0QX65	gene bioF	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pimeloyl-[acyl-carrier protein] + L-alanine	-	Mycolicibacterium smegmatis	8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]	-	?
pimeloyl-[acyl-carrier protein] + L-alanine	-	Mycolicibacterium smegmatis ATCC 700084	8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]	-	?

Subunits

Subunits	Comment	Organism
dimer	the structure reveals an incomplete dimer, three-dimensional structure of apoenzyme, overview	Mycolicibacterium smegmatis

Synonyms

Synonyms	Comment	Organism
7-keto-8-aminopelargonic acid synthase	-	Mycolicibacterium smegmatis
BioF	-	Mycolicibacterium smegmatis
KAPA synthase	-	Mycolicibacterium smegmatis

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	dependent on, binding site residues His129, Lys235, and His200 are essential for MsBioF activity in vivo	Mycolicibacterium smegmatis

General Information

General Information	Comment	Organism
metabolism	the enzyme is essential for mycobacterial growth and plays a key role as the gatekeeper of the biotin biosynthesis pathway, catalyzing the first committed step, decarboxylative condensation of L-alanine with pimeloyl-CoA to form 7-oxo-8-aminopelargonic acid	Mycolicibacterium smegmatis
additional information	the structure reveals an incomplete dimer, and the active site organization is similar to, but distinct from Escherichia coli 8-amino-7-oxononanoate synthase (EcAONS). Pyridoxal 5'-phosphate binding site residues His129, Lys235, and His200 are essential for MsBioF activity in vivo and residue Glu171 plays an important, but not essential role in MsBioF activity. The N-terminus (residues 1-37) is also essential for MsBioF activity in vivo, structure homology modeling and structure-function analysis of MsBioF, overview	Mycolicibacterium smegmatis

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Release 2023.1 (January 2023)