Cloned (Comment) | Organism |
---|---|
expression of wild-type Sas3 protein and mutants as GST-fusion proteins in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
C303A | site-directed mutagenesis of Sas3, exchange in zinc finger motif, remaining activity: 4.5% of wild-type activity | Saccharomyces cerevisiae |
C306A | site-directed mutagenesis of Sas3, exchange in zinc finger motif, remaining activity: 11.1% of wild-type activity | Saccharomyces cerevisiae |
C323A | site-directed mutagenesis of Sas3, exchange in zinc finger motif, no remaining activity | Saccharomyces cerevisiae |
G429E | site-directed mutagenesis of Sas3, exchange in acetyl-CoA binding motif, no remaining activity | Saccharomyces cerevisiae |
G431A | site-directed mutagenesis of Sas3, exchange in acetyl-CoA binding motif, nearly no remaining activity: 0.8% of wild-type activity | Saccharomyces cerevisiae |
H319A | site-directed mutagenesis of Sas3, exchange in zinc finger motif, nearly no remaining activity: 1.2% of wild-type activity | Saccharomyces cerevisiae |
K428A | site-directed mutagenesis of Sas3, exchange in acetyl-CoA binding motif, remaining activity: 55.9% of wild-type activity | Saccharomyces cerevisiae |
Q426A | site-directed mutagenesis of Sas3, exchange in acetyl-CoA binding motif, remaining activity: 38.6% of wild-type activity | Saccharomyces cerevisiae |
Y430A | site-directed mutagenesis of Sas3, exchange in acetyl-CoA binding motif, remaining activity: 16.7% of wild-type activity | Saccharomyces cerevisiae |
Y430L | site-directed mutagenesis of Sas3, exchange in acetyl-CoA binding motif, remaining activity: 87.0% of wild-type activity | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
Sas3 gene | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type Sas3 protein and mutants as GST-fusion proteins from E. coli | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + histone | - |
Saccharomyces cerevisiae | CoA + acetylhistone | - |
? | |
acetyl-CoA + histone H2A | - |
Saccharomyces cerevisiae | CoA + acetylhistone H2A | - |
? | |
acetyl-CoA + histone H3 | - |
Saccharomyces cerevisiae | CoA + acetylhistone H3 | - |
? | |
acetyl-CoA + histone H4 | histone H4 is the preferred substrate | Saccharomyces cerevisiae | CoA + acetylhistone H4 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HAT | - |
Saccharomyces cerevisiae |
More | enzymes with histone acetyltransferase and transcription cofactor activity belong to the MYST protein family | Saccharomyces cerevisiae |