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Literature summary for 2.3.1.48 extracted from

  • Takechi, S.; Nakayama, T.
    Sas3 is a histone acetyltransferase and requires a zinc finger motif (1999), Biochem. Biophys. Res. Commun., 266, 405-410.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type Sas3 protein and mutants as GST-fusion proteins in Escherichia coli Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
C303A site-directed mutagenesis of Sas3, exchange in zinc finger motif, remaining activity: 4.5% of wild-type activity Saccharomyces cerevisiae
C306A site-directed mutagenesis of Sas3, exchange in zinc finger motif, remaining activity: 11.1% of wild-type activity Saccharomyces cerevisiae
C323A site-directed mutagenesis of Sas3, exchange in zinc finger motif, no remaining activity Saccharomyces cerevisiae
G429E site-directed mutagenesis of Sas3, exchange in acetyl-CoA binding motif, no remaining activity Saccharomyces cerevisiae
G431A site-directed mutagenesis of Sas3, exchange in acetyl-CoA binding motif, nearly no remaining activity: 0.8% of wild-type activity Saccharomyces cerevisiae
H319A site-directed mutagenesis of Sas3, exchange in zinc finger motif, nearly no remaining activity: 1.2% of wild-type activity Saccharomyces cerevisiae
K428A site-directed mutagenesis of Sas3, exchange in acetyl-CoA binding motif, remaining activity: 55.9% of wild-type activity Saccharomyces cerevisiae
Q426A site-directed mutagenesis of Sas3, exchange in acetyl-CoA binding motif, remaining activity: 38.6% of wild-type activity Saccharomyces cerevisiae
Y430A site-directed mutagenesis of Sas3, exchange in acetyl-CoA binding motif, remaining activity: 16.7% of wild-type activity Saccharomyces cerevisiae
Y430L site-directed mutagenesis of Sas3, exchange in acetyl-CoA binding motif, remaining activity: 87.0% of wild-type activity Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
Sas3 gene
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type Sas3 protein and mutants as GST-fusion proteins from E. coli Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + histone
-
Saccharomyces cerevisiae CoA + acetylhistone
-
?
acetyl-CoA + histone H2A
-
Saccharomyces cerevisiae CoA + acetylhistone H2A
-
?
acetyl-CoA + histone H3
-
Saccharomyces cerevisiae CoA + acetylhistone H3
-
?
acetyl-CoA + histone H4 histone H4 is the preferred substrate Saccharomyces cerevisiae CoA + acetylhistone H4
-
?

Synonyms

Synonyms Comment Organism
HAT
-
Saccharomyces cerevisiae
More enzymes with histone acetyltransferase and transcription cofactor activity belong to the MYST protein family Saccharomyces cerevisiae