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Literature summary for 2.3.1.48 extracted from

  • Poux, A.N.; Cebrat, M.; Kim, C.M.; Cole, P.A.; Marmorstein, R.
    Structure of the GCN5 histone acetyltransferase bound to a bisubstrate inhibitor (2002), Proc. Natl. Acad. Sci. USA, 99, 14065-14070.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.48

Cloned(Commentary)

Cloned (Comment) Organism
expression of the catalytic domain of PCAF protein, amino acid residues 493-658, in Escherichia coli BL21 (DE3) Homo sapiens
overexpression of enzyme domain tGcn5 consisting of amino acid residues 48-210 in Escherichia coli 834 (DE3) Tetrahymena thermophila

Crystallization (Commentary)

Crystallization (Comment) Organism
tGcn5 domain cocrystallized with inhibitor histone H3-methyl-CoA-peptide of 20 amino acid residues, hanging drop vapour diffusion method at 20°C, 2.0 M (NH4)2SO4, 0.1 M Na cacodylate, pH 6.6, 0.2 M NaCl, CoA is bound via isopropionyl linker to Lys14, structure analysis, modeling of conformational changes Tetrahymena thermophila

Protein Variants

Protein Variants Comment Organism
Y638A mutant of PCAF catalytic domain, increased Km and decreased kcat compared to wild-type Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
bisubstrate analogue histone H3-peptide-coenzyme A peptide consisting of 20 and 7 amino acid residues Homo sapiens
bisubstrate analogue histone H3-peptide-coenzyme A
-
 Tetrahymena thermophila
bisubstrate analogue methyl-histone H3-peptide-coenzyme A peptide consisting of 20 and 7 amino acid residues Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
 acetyl-CoA recombinant PCAF catalytic domain mutant Y638A Homo sapiens
0.0016
-
 acetyl-CoA recombinant wild-type PCAF catalytic domain Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 GCN5
-
Homo sapiens GCN5
-
 GCN5
-
Tetrahymena thermophila
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged catalytic domain of PCAF protein from E. coli Homo sapiens
tGcn5 domain, recombinant from E. coli Tetrahymena thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + histone
-
 Homo sapiens CoA + acetylhistone
-
 ?
acetyl-CoA + histone
-
 Tetrahymena thermophila CoA + acetylhistone
-
 ?
acetyl-CoA + histone
-
 Homo sapiens GCN5 CoA + acetylhistone
-
 ?
acetyl-CoA + histone H3 peptide peptide H3p20 Homo sapiens CoA + acetylhistone H3 peptide
-
 ?
acetyl-CoA + histone H3 peptide peptide H3p20 Tetrahymena thermophila CoA + acetylhistone H3 peptide
-
 ?
acetyl-CoA + histone H3 peptide peptide H3p20 Homo sapiens GCN5 CoA + acetylhistone H3 peptide
-
 ?
acetyl-CoA + histone H3-peptide p20
-
 Homo sapiens CoA + acetylhistone H3 -peptide p20
-
 ?
acetyl-CoA + histone H3-peptide p20
-
 Homo sapiens GCN5 CoA + acetylhistone H3 -peptide p20
-
 ?

Synonyms

Synonyms Comment Organism
HAT
-
 Homo sapiens
HAT
-
 Tetrahymena thermophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 PCAF protein, assay at Homo sapiens
30
-
 tGcn5 domain Tetrahymena thermophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00317
-
 histone H3-peptide p20 PCAF catalytic domain, recombinant mutant Y638A Homo sapiens
0.0583
-
 histone H3-peptide p20 PCAF catalytic domain, recombinant wild-type Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens
8
-
 assay at Tetrahymena thermophila
8
-
 tGnc5 domain Tetrahymena thermophila
8
-
 PCAF protein Homo sapiens