Protein Variants | Comment | Organism |
---|---|---|
C304A | site-directed mutagenesis, the mutant shows activity and pH-dependence similar to the wild-type enzyme | Saccharomyces cerevisiae |
C304S | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Saccharomyces cerevisiae |
E338Q | site-directed mutagenesis, the mutant shows 200fold reduced kcat at pH 7.5 compared to the wild-type enzyme | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetic analyses, kinetic mechanism | Saccharomyces cerevisiae | |
0.0012 | - |
acetyl-CoA | pH 7.5, 25°C, mutant E338Q | Saccharomyces cerevisiae | |
0.0015 | - |
acetyl-CoA | pH 7.5, 25°C, mutant C304A | Saccharomyces cerevisiae | |
0.002 | - |
acetyl-CoA | pH 7.5, 25°C, mutant C304S | Saccharomyces cerevisiae | |
0.0025 | - |
acetyl-CoA | pH 7.5, 25°C, wild-type enzyme | Saccharomyces cerevisiae | |
0.135 | - |
piccoloNuA4 peptide | pH 7.5, 25°C, mutant E338Q | Saccharomyces cerevisiae | |
0.182 | - |
piccoloNuA4 peptide | pH 7.5, 25°C, mutant C304S | Saccharomyces cerevisiae | |
0.216 | - |
piccoloNuA4 peptide | pH 7.5, 25°C, wild-type enzyme | Saccharomyces cerevisiae | |
0.372 | - |
piccoloNuA4 peptide | pH 7.5, 25°C, mutant C304A | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
histone + acetyl-CoA | Saccharomyces cerevisiae | - |
acetyl-histone + CoA | - |
? | |
piccoloNuA4 peptide + acetyl-CoA | Saccharomyces cerevisiae | the peptide is part of the physiologic enzme complex, overview | acetyl-piccoloNuA4 peptide + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q08649 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine | ordered sequential catalytic mechanism of the MYST HAT with a direct-attack mechanism and direct acetyl transfer mechanism, not mediated by Cys304, within an Esa1acetyl-CoAhistone ternary complex, acetyl-CoA binds first and CoA is the last product released | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
histone + acetyl-CoA | - |
Saccharomyces cerevisiae | acetyl-histone + CoA | - |
? | |
histone + propionyl-CoA | - |
Saccharomyces cerevisiae | propionyl-histone + CoA | - |
? | |
histone H4 + acetyl-CoA | - |
Saccharomyces cerevisiae | acetyl-histone H4 + CoA | - |
? | |
additional information | in the absence of histone acceptor, slow rates of enzyme autoacetylation and of CoA formation occur | Saccharomyces cerevisiae | ? | - |
? | |
piccoloNuA4 peptide + acetyl-CoA | the peptide is part of the physiologic enzme complex, overview | Saccharomyces cerevisiae | acetyl-piccoloNuA4 peptide + CoA | - |
? | |
piccoloNuA4 peptide + propionyl-CoA | - |
Saccharomyces cerevisiae | propionyl-piccoloNuA4 peptide + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Esa1 | - |
Saccharomyces cerevisiae |
HAT | - |
Saccharomyces cerevisiae |
More | the enzyme belongs to the MYST family histone acetyltransferase | Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0091 | - |
acetyl-CoA | pH 7.5, 25°C, mutant E338Q | Saccharomyces cerevisiae | |
0.14 | - |
acetyl-CoA | pH 7.5, 25°C, mutant C304S | Saccharomyces cerevisiae | |
0.76 | - |
acetyl-CoA | pH 7.5, 25°C, mutant C304A | Saccharomyces cerevisiae | |
1.6 | - |
acetyl-CoA | pH 7.5, 25°C, wild-type enzyme | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH profiles of wild-type and mutant enzymes | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Saccharomyces cerevisiae |