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Literature summary for 2.3.1.48 extracted from
- TAF1 histone acetyltransferase activity in Sp1 activation of the cyclin D1 promoter (2005), Mol. Cell. Biol., 25, 4321-4332.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzymes in Spodoptera frugiperda Sf9 cells using the baculovirus expression system, genetic regulation of enzyme expression, overview | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C746A | site-directed mutagenesis, mutation in the HAT domain, leads to only slightly reduced catalytic activity, but highly reduced cyclin D1 transcription in the cells | Homo sapiens |
| E742Q | site-directed mutagenesis, mutation in the HAT domain, leads to only slightly reduced catalytic activity, but highly reduced cyclin D1 transcription in the cells | Homo sapiens |
| G923/925D | site-directed mutagenesis, mutation in the HAT domain, leads to only slightly reduced catalytic activity, but highly reduced cyclin D1 transcription in the cells | Homo sapiens |
| additional information | missense mutation within the histone acetyltransferase domain of the TATA binding protein associated factor TAF1 induces ts13 cells to undergo a late G1 arrest and decreases cyclin D1 transcription, construction of several deletion mutants, some of which show slightly reduced activity, while deletion mutants DELTA844-850 and DELTA848-850 are deficient in HAT activity and unable to complement the ts13 defect in cell proliferation and cyclin D1 transcription, hypoacetylation of H3 at the cyclin D1 promoter upon inactivation of TAF1 in ts13 cells, regulation of gene expressions in the pathway, overview | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| histone H3 + acetyl-CoA | Homo sapiens | - |
acetyl-histone H3 + CoA | - |
? |  |
| additional information | Homo sapiens | the enzyme is involved in Sp1 activation of the cyclin D1 promoter, TAF1-dependent histone acetylation facilitates transcription factor binding to the Sp1 sites, thereby activating cyclin D1 transcription and ultimately G1-to-S-phase progression, regulation, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
gene taf1 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| histone H3 + acetyl-CoA | - |
Homo sapiens | acetyl-histone H3 + CoA | - |
? | |
| additional information | the enzyme is involved in Sp1 activation of the cyclin D1 promoter, TAF1-dependent histone acetylation facilitates transcription factor binding to the Sp1 sites, thereby activating cyclin D1 transcription and ultimately G1-to-S-phase progression, regulation, overview | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HAT | - |
Homo sapiens |
| TAF1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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