Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.5 extracted from

  • Walraven, J.M.; Trent, J.O.; Hein, D.W.
    Computational and eperimental aalyses of mammalian arylamine N-acetyltransferase structure and function (2007), Drug Metab. Dispos., 35, 1001-1007.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
into the pKK223-3 bacterial expression vector for transformation of Escherichia coli JM105 cells Homo sapiens
isozyme NAT2, DNA and amino acid sequence determination and analysis, expression of wild-type and mutants in Escherichia coli strain JM105 Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of several insertion and deletion mutants of NAT2, overview Homo sapiens
additional information ANDH, human NAT2 mutant, a 17-residue segment is deleted and replaced Homo sapiens
additional information GG, human NAT2 mutant, a 17-residue segment is deleted and replaced Homo sapiens
additional information GGSG, human NAT2 mutant, a 17-residue segment is deleted and replaced Homo sapiens
additional information GRSG, human NAT2 mutant, a 17-residue segment is deleted and replaced Homo sapiens
additional information HHEH, human NAT2 mutant, a 17-residue segment is deleted and replaced Homo sapiens
additional information PHAG, human NAT2 mutant, a 17-residue segment is deleted and replaced Homo sapiens
additional information QEGST, human NAT2 mutant, a 17-residue segment is deleted and replaced Homo sapiens
additional information SDGSD, human NAT2 mutant, a 17-residue segment is deleted and replaced Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + an arylamine Homo sapiens the enzyme is important for the activation and deactivation of exocyclic amine-containing pro-carcinogens, and for the metabolism of some pharmaceutical drugs CoA + an N-acetylarylamine
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens P11245 isozyme NAT2; isozyme NAT2
-
Homo sapiens P18440 isozyme NAT1; isozyme NAT1
-

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine structure-function analysis, Cys68-His107-Asp122 catalytic triad Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
additional information wide tissue distribution Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0005
-
mutant GGSG Homo sapiens
0.0005
-
mutant HHEH Homo sapiens
0.0006
-
mutant GGSG Homo sapiens
0.0006
-
mutant HHEH Homo sapiens
0.0013
-
mutant GRSG Homo sapiens
0.0014
-
mutant GRSG Homo sapiens
0.0055
-
mutant GG Homo sapiens
0.006
-
mutant GG Homo sapiens
0.0061
-
-
Homo sapiens
0.0064
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + an arylamine the enzyme is important for the activation and deactivation of exocyclic amine-containing pro-carcinogens, and for the metabolism of some pharmaceutical drugs Homo sapiens CoA + an N-acetylarylamine
-
?
acetyl-CoA + an arylamine the enzyme catalyzes the N-acetylation of arylamines and hydrazines and O-acetylation of N-hydroxy-arylamines and heterocyclic amines Homo sapiens CoA + an N-acetylarylamine
-
?
acetyl-CoA + sulfamethazine
-
Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
More secondary structures of wild-type and mutant enzymes, structure-function analysis, computational docking of NAT substrates, NAT homology modeling based on crystal structures of bacteria with Protein Data Bank accession numbers 1E2T, 1W4T, 1GX3, and 2BSZ, overview Homo sapiens

Synonyms

Synonyms Comment Organism
arylamine N-acetyltransferase
-
Homo sapiens
NAT
-
Homo sapiens
NAT2
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens
37
-
activity assay Homo sapiens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 control Homo sapiens
30 33 mutant GG Homo sapiens

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
Homo sapiens