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Literature summary for 2.3.1.5 extracted from

  • Liu, F.; Zhang, N.; Zhou, X.; Hanna, P.E.; Wagner, C.R.; Koepp, D.M.; Walters, K.J.
    Arylamine N-acetyltransferase aggregation and constitutive ubiquitylation (2006), J. Mol. Biol., 361, 482-492.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of FLAG-tagged wild-type and mutant NAT2 in HEK-293T cells Mesocricetus auratus
expression of wild-type and mutant NAT1 in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information humans harboring certain genetic variations within the NAT genes exhibit increased likelihood of developing various cancer types, especially urinary bladder cancer, polymorphisms, the mutants exhibit reduced cellular activity, which is proposed to be due to their constitutive ubiquitylation and enhanced proteasomal degradation, overview Homo sapiens
R64W naturally occuring polymorphism in gene NAT1, the R64W mutation also causes constitutive ubiquitinylation and NAT1 to aggregate in cultured cells, does not interfere with NAT catalysis in vitro, overall protein structure and thermostability are not compromised Homo sapiens
R64W naturally occuring polymorphism in gene NAT2, the R64W mutation also causes constitutive ubiquitinylation and NAT2 to aggregate in cultured cells, does not interfere with NAT catalysis in vitro, overall protein structure and thermostability are not compromised Mesocricetus auratus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
4-Aminobenzoic acid pH 7.0, 25°C, recombinant wild-type enzyme Mesocricetus auratus
0.37
-
4-Aminobenzoic acid pH 7.0, 25°C, recombinant mutant R64W Mesocricetus auratus
4.81
-
4-nitrophenyl acetate pH 7.0, 25°C, recombinant mutant R64W Mesocricetus auratus
10
-
4-nitrophenyl acetate pH 7.0, 25°C, recombinant wild-type enzyme Mesocricetus auratus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mesocricetus auratus isozyme NAT1 acetylates and detoxifies arylamine carcinogens ?
-
?
additional information Homo sapiens isozyme NAT1 acetylates and detoxifies arylamine carcinogens ?
-
?
additional information Mesocricetus auratus isozyme NAT2 acetylates and detoxifies arylamine carcinogens ?
-
?
additional information Homo sapiens isozyme NAT2 acetylates and detoxifies arylamine carcinogens ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P11245 isozyme NAT2; isozyme NAT2
-
Homo sapiens P18440 isozyme NAT1; isozyme NAT1
-
Mesocricetus auratus P50292 isozyme NAT1; isozyme NAT1
-
Mesocricetus auratus P50293 isozyme NAT2; isozyme NAT2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes NAT2 from HEK-293T cells by affinity chromatography Mesocricetus auratus
recombinant wild-type and mutant NAT1 from Escherichia coli Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Mesocricetus auratus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + 4-aminobenzoic acid
-
Mesocricetus auratus 4-nitrophenol + N-acetyl-4-aminobenzoic acid
-
?
additional information isozyme NAT1 acetylates and detoxifies arylamine carcinogens Mesocricetus auratus ?
-
?
additional information isozyme NAT1 acetylates and detoxifies arylamine carcinogens Homo sapiens ?
-
?
additional information isozyme NAT2 acetylates and detoxifies arylamine carcinogens Mesocricetus auratus ?
-
?
additional information isozyme NAT2 acetylates and detoxifies arylamine carcinogens Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
NAT
-
Mesocricetus auratus
NAT
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Mesocricetus auratus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
56.3
-
half-life of the acetylated state of recombinant R64W NAT at 25 °C, pH 7.0, cooperative unfolding is lost for the hamster NAT2 R64W variant Mesocricetus auratus
56.3
-
half-life of the acetylated state of recombinant R64W NAT2 at 25 °C, pH 7.0, cooperative unfolding is lost for the hamster NAT2 R64W variant Mesocricetus auratus
88.3
-
half-life of the acetylated state of recombinant wild-type NAT at 25 °C, pH 7.0, temperature-dependent unfolding of the wild-type enzyme is highly cooperative Mesocricetus auratus
88.3
-
half-life of the acetylated state of recombinant wild-type NAT2 at 25 °C, pH 7.0, temperature-dependent unfolding of the wild-type enzyme is highly cooperative Mesocricetus auratus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
265
-
4-Aminobenzoic acid pH 7.0, 25°C, recombinant mutant R64W, with 4-nitrophenyl acetate Mesocricetus auratus
265
-
4-Aminobenzoic acid pH 7.0, 25°C, recombinant wild-type enzyme, with 4-nitrophenyl acetate Mesocricetus auratus
620
-
4-nitrophenyl acetate pH 7.0, 25°C, recombinant mutant R64W, with 4-aminobenzoic acid Mesocricetus auratus
620
-
4-nitrophenyl acetate pH 7.0, 25°C, recombinant wild-type enzyme, with 4-aminobenzoic acid Mesocricetus auratus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Mesocricetus auratus