Cloned (Comment) | Organism |
---|---|
expression of FLAG-tagged wild-type and mutant NAT2 in HEK-293T cells | Mesocricetus auratus |
expression of wild-type and mutant NAT1 in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | humans harboring certain genetic variations within the NAT genes exhibit increased likelihood of developing various cancer types, especially urinary bladder cancer, polymorphisms, the mutants exhibit reduced cellular activity, which is proposed to be due to their constitutive ubiquitylation and enhanced proteasomal degradation, overview | Homo sapiens |
R64W | naturally occuring polymorphism in gene NAT1, the R64W mutation also causes constitutive ubiquitinylation and NAT1 to aggregate in cultured cells, does not interfere with NAT catalysis in vitro, overall protein structure and thermostability are not compromised | Homo sapiens |
R64W | naturally occuring polymorphism in gene NAT2, the R64W mutation also causes constitutive ubiquitinylation and NAT2 to aggregate in cultured cells, does not interfere with NAT catalysis in vitro, overall protein structure and thermostability are not compromised | Mesocricetus auratus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
4-Aminobenzoic acid | pH 7.0, 25°C, recombinant wild-type enzyme | Mesocricetus auratus | |
0.37 | - |
4-Aminobenzoic acid | pH 7.0, 25°C, recombinant mutant R64W | Mesocricetus auratus | |
4.81 | - |
4-nitrophenyl acetate | pH 7.0, 25°C, recombinant mutant R64W | Mesocricetus auratus | |
10 | - |
4-nitrophenyl acetate | pH 7.0, 25°C, recombinant wild-type enzyme | Mesocricetus auratus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mesocricetus auratus | isozyme NAT1 acetylates and detoxifies arylamine carcinogens | ? | - |
? | |
additional information | Homo sapiens | isozyme NAT1 acetylates and detoxifies arylamine carcinogens | ? | - |
? | |
additional information | Mesocricetus auratus | isozyme NAT2 acetylates and detoxifies arylamine carcinogens | ? | - |
? | |
additional information | Homo sapiens | isozyme NAT2 acetylates and detoxifies arylamine carcinogens | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P11245 | isozyme NAT2; isozyme NAT2 | - |
Homo sapiens | P18440 | isozyme NAT1; isozyme NAT1 | - |
Mesocricetus auratus | P50292 | isozyme NAT1; isozyme NAT1 | - |
Mesocricetus auratus | P50293 | isozyme NAT2; isozyme NAT2 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes NAT2 from HEK-293T cells by affinity chromatography | Mesocricetus auratus |
recombinant wild-type and mutant NAT1 from Escherichia coli | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Mesocricetus auratus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + 4-aminobenzoic acid | - |
Mesocricetus auratus | 4-nitrophenol + N-acetyl-4-aminobenzoic acid | - |
? | |
additional information | isozyme NAT1 acetylates and detoxifies arylamine carcinogens | Mesocricetus auratus | ? | - |
? | |
additional information | isozyme NAT1 acetylates and detoxifies arylamine carcinogens | Homo sapiens | ? | - |
? | |
additional information | isozyme NAT2 acetylates and detoxifies arylamine carcinogens | Mesocricetus auratus | ? | - |
? | |
additional information | isozyme NAT2 acetylates and detoxifies arylamine carcinogens | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAT | - |
Mesocricetus auratus |
NAT | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mesocricetus auratus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
56.3 | - |
half-life of the acetylated state of recombinant R64W NAT at 25 °C, pH 7.0, cooperative unfolding is lost for the hamster NAT2 R64W variant | Mesocricetus auratus |
56.3 | - |
half-life of the acetylated state of recombinant R64W NAT2 at 25 °C, pH 7.0, cooperative unfolding is lost for the hamster NAT2 R64W variant | Mesocricetus auratus |
88.3 | - |
half-life of the acetylated state of recombinant wild-type NAT at 25 °C, pH 7.0, temperature-dependent unfolding of the wild-type enzyme is highly cooperative | Mesocricetus auratus |
88.3 | - |
half-life of the acetylated state of recombinant wild-type NAT2 at 25 °C, pH 7.0, temperature-dependent unfolding of the wild-type enzyme is highly cooperative | Mesocricetus auratus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
265 | - |
4-Aminobenzoic acid | pH 7.0, 25°C, recombinant mutant R64W, with 4-nitrophenyl acetate | Mesocricetus auratus | |
265 | - |
4-Aminobenzoic acid | pH 7.0, 25°C, recombinant wild-type enzyme, with 4-nitrophenyl acetate | Mesocricetus auratus | |
620 | - |
4-nitrophenyl acetate | pH 7.0, 25°C, recombinant mutant R64W, with 4-aminobenzoic acid | Mesocricetus auratus | |
620 | - |
4-nitrophenyl acetate | pH 7.0, 25°C, recombinant wild-type enzyme, with 4-aminobenzoic acid | Mesocricetus auratus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Mesocricetus auratus |