Cloned (Comment) | Organism |
---|---|
expression of His-tagged isozyme NAT1 in Escherichia coli strain BL21(DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydrogen peroxide | inactivates isozyme NAT1, in vivo effect, overview | Homo sapiens | |
peroxynitrite | inactivates isozyme NAT1, in vivo effect, overview | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + an arylamine | Homo sapiens | NATs are xenobiotic metabolizing enzymes responsible for the acetylation of many arylamine and heterocyclic amines, they therefore play an important role in the detoxification and activation of numerous drugs and carcinogens, regulation, overview | CoA + an N-acetylarylamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P11245 | isozyme NAT2; isozyme NAT2 | - |
Homo sapiens | P18440 | isozyme NAT1; isozyme NAT1 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozyme NAT1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestine | isozyme NAT2 mainly | Homo sapiens | - |
liver | isozyme NAT2 mainly | Homo sapiens | - |
MCF-7 cell | mammary carcinoma cell line, NAT1 | Homo sapiens | - |
additional information | isozyme NAT1 shows a broad tissue distribution | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + 5-aminosalicylic acid | - |
Homo sapiens | 4-nitrophenol + 5-acetylaminosalicylic acid | - |
? | |
acetyl-CoA + 4-dimethylaminobenzaldehyde | - |
Homo sapiens | CoA + 5-acetyl-4-dimethylaminobenzaldehyde | - |
? | |
acetyl-CoA + 5-aminosalicylic acid | - |
Homo sapiens | CoA + 5-acetylaminosalicylic acid | - |
? | |
acetyl-CoA + an arylamine | - |
Homo sapiens | CoA + an N-acetylarylamine | - |
? | |
acetyl-CoA + an arylamine | NATs are xenobiotic metabolizing enzymes responsible for the acetylation of many arylamine and heterocyclic amines, they therefore play an important role in the detoxification and activation of numerous drugs and carcinogens, regulation, overview | Homo sapiens | CoA + an N-acetylarylamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAT | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inactivation kinetics of NAT1 by oxidants, overview | Homo sapiens |