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Literature summary for 2.3.1.5 extracted from
- Cloning and molecular characterization of three arylamine N-acetyltransferase genes from Bacillus anthracis: identification of unusual enzymatic properties and their contribution to sulfamethoxazole resistance (2007), Biochemistry, 46, 7069-7078.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Bacillus anthracis |
| into the pET28a vector for expression in Escherichia coli BL21DE3 pLysS cells | Bacillus anthracis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C69A | BanatC inactive mutant, replacement of the catalytic cysteine residue | Bacillus anthracis |
| C69A | mutant devoid of NAT activity. Expression of the C69A mutant in Escherichia coli does not afford higher-than-normal resistance to sulfonamide antibiotic sulfamethoxazole in the recombinant bacteria | Bacillus anthracis |
| additional information | expression of BanatC in Escherichia coli affords higher-than-normal resistance to sulfonamide antibiotic sulfamethoxazole in the recombinant bacteria | Bacillus anthracis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.084 | - |
2-Aminofluorene | apparent KM | Bacillus anthracis |  |
| 0.084 | - |
2-Aminofluorene | isoenzyme BanatB | Bacillus anthracis | |
| 0.21 | - |
2-Aminofluorene | apparent KM | Bacillus anthracis | |
| 0.21 | - |
2-Aminofluorene | isoenzyme BanatC | Bacillus anthracis | |
| 0.228 | - |
5-Aminosalicylate | apparent KM | Bacillus anthracis | |
| 0.228 | - |
5-Aminosalicylate | isoenzyme BanatC | Bacillus anthracis | |
| 0.622 | - |
5-Aminosalicylate | apparent KM | Bacillus anthracis | |
| 0.622 | - |
5-Aminosalicylate | isoenzyme BanatB | Bacillus anthracis | |
| 0.662 | - |
sulfamethoxazole | apparent KM | Bacillus anthracis | |
| 0.662 | - |
sulfamethoxazole | isoenzyme BanatC | Bacillus anthracis | |
| 0.834 | - |
4-Aminosalicylate | apparent KM | Bacillus anthracis | |
| 0.834 | - |
4-Aminosalicylate | isoenzyme BanatC | Bacillus anthracis | |
| 0.849 | - |
hydralazine | apparent KM | Bacillus anthracis | |
| 0.849 | - |
hydralazine | isoenzyme BanatB | Bacillus anthracis | |
| 1.327 | - |
hydralazine | apparent KM | Bacillus anthracis | |
| 1.327 | - |
hydralazine | isoenzyme BanatC | Bacillus anthracis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
His-tagged BanatA, determined by SDS-PAGE and Western blot analysis | Bacillus anthracis |
| 27000 | - |
SDS-PAGE isoenzyme BanatA | Bacillus anthracis |
| 31000 | - |
His-tagged BanatB, determined by SDS-PAGE and Western blot analysis | Bacillus anthracis |
| 31000 | - |
SDS-PAGE isoenzyme BanatB | Bacillus anthracis |
| 34000 | - |
His-tagged BanatC, determined by SDS-PAGE and Western blot analysis | Bacillus anthracis |
| 34000 | - |
SDS-PAGE, isoenzyme BanatC | Bacillus anthracis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus anthracis | A0A0F7R932 | - |
- |
| Bacillus anthracis | A0A0F7R932 | BanatA | - |
| Bacillus anthracis | Q81PT0 | - |
- |
| Bacillus anthracis | Q81PT0 | BanatB | - |
| Bacillus anthracis | Q81R98 | - |
- |
| Bacillus anthracis | Q81R98 | BanatC | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using a HIS-Select nickel resin, CM Sephadex ion exchange chromatography | Bacillus anthracis |
| using HIS-select nickel resin and CM Sephadex ion exchange chromatography, in addition protein extracts from stationary phase cultures of Bacillus anthracis are preapred | Bacillus anthracis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
BanatA is devoid of NAT or AcCoA/p-nitrophenyl acetate hydrolysis activities, suggesting that it may be a new bacterial NAT-like protein with unknown function | Bacillus anthracis |
| additional information | - |
no activity with substrates: isoniazid, 4-aminobenzoic acid, isoenzyme BanatC | Bacillus anthracis |
| additional information | - |
substrate: sulfamethoxazolea, 4-aminobenzoic, 4-aminosalicylate, isoniazid acid no activity detected | Bacillus anthracis |
| 0.168 | - |
- |
Bacillus anthracis |
| 0.168 | - |
enzymatic activity detected in the absence of an arylamine substrate: BanatB, but not BanatC, displays AcCoA and /or p-nitrophenyl acetate hydrolysis activity in the absence of an arylamine substrate | Bacillus anthracis |
| 0.416 | - |
substrate: sulfamethoxazole, isoenzyme BanatC | Bacillus anthracis |
| 0.416 | - |
sulfamethoxazole | Bacillus anthracis |
| 0.603 | - |
2-aminofluorene | Bacillus anthracis |
| 0.603 | - |
substrate: 2-aminofluorenea | Bacillus anthracis |
| 0.969 | - |
hydralazine | Bacillus anthracis |
| 0.969 | - |
substrate: hydralazine | Bacillus anthracis |
| 1.36 | - |
5-aminosalicylate | Bacillus anthracis |
| 1.36 | - |
substrate: 5-aminosalicylate | Bacillus anthracis |
| 1.744 | - |
hydralazine | Bacillus anthracis |
| 1.744 | - |
substrate: hydralazine, isoenzyme BanatC | Bacillus anthracis |
| 3.109 | - |
4-aminosalicylate | Bacillus anthracis |
| 3.109 | - |
substrate: 4-aminosalicylate, isoenzyme BanatC | Bacillus anthracis |
| 3.679 | - |
5-aminosalicylate | Bacillus anthracis |
| 3.679 | - |
substrate: 5-aminosalicylate, isoenzyme BanatC | Bacillus anthracis |
| 5.852 | - |
2-aminofluorene | Bacillus anthracis |
| 5.852 | - |
substrate: 2-aminofluorene, isoenzyme BanatC | Bacillus anthracis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + 2-aminofluorene | - |
Bacillus anthracis | CoA + N-acetyl-2-aminofluorene | - |
? | |
| acetyl-CoA + 4-aminosalicylate | - |
Bacillus anthracis | CoA + N-acetyl-4-aminosalicylate | - |
? | |
| acetyl-CoA + 5-aminosalicylate | - |
Bacillus anthracis | CoA + N-acetyl-5-aminosalicylate | - |
? | |
| acetyl-CoA + hydralazine | - |
Bacillus anthracis | ? | - |
? | |
| acetyl-CoA + hydralazine | - |
Bacillus anthracis | CoA + N-acetyl-hydralazine | - |
? | |
| acetyl-CoA + sulfamethoxazole | - |
Bacillus anthracis | CoA + N-acetyl-sulfamethoxazole | - |
? | |
| acetyl-CoA + sulfamethoxazole | - |
Bacillus anthracis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| arylamine N-acetyltransferase | - |
Bacillus anthracis |
| BanatA | - |
Bacillus anthracis |
| BanatB | - |
Bacillus anthracis |
| BanatB | in contrast to BanatA or BanatC, BanatB also has acetyl-CoA and p-nitrophenyl acetate hydrolysis activity in the absence of arylamine substrates, indicating that it may act also as an AcCoA hydrolase | Bacillus anthracis |
| BanatC | - |
Bacillus anthracis |
| NAT | - |
Bacillus anthracis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bacillus anthracis |
| 37 | - |
activity assay | Bacillus anthracis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Bacillus anthracis |
| 7.5 | - |
activity assay | Bacillus anthracis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Bacillus anthracis | |
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