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Literature summary for 2.3.1.5 extracted from

  • Wu, H.; Dombrovsky, L.; Tempel, W.; Martin, F.; Loppnau, P.; Goodfellow, G.H.; Grant, D.M.; Plotnikov, A.N.
    Structural basis of substrate-binding specificity of human arylamine N-acetyltransferases (2007), J. Biol. Chem., 282, 30189-30197.
    View publication on PubMed
Search for more literature for 2.3.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
high resolution crystal structures of both human NATs, including NAT1 in complex with the irreversible inhibitor 2-bromoacetanilide, a site-directed NAT1 mutant, NAT1-F125S, and a NAT2-CoA complex are presented. By comparing the structures with known prokaryotic structures, evidences are provided for novel structural features of human NATs that are absent in bacterial enzymes, including an insertion that produces a significant difference in the structure of the carboxyl terminus of the eukaryotic enzymes Homo sapiens
high resolution crystal structures of both human NATs, including NAT1 in complex with the irreversible inhibitor 2-bromoacetanilide, a site-directed NAT1 mutant, NAT1-F125S, and a NAT2-CoA complex are presented. By comparing the structures with known prokaryotic structures, evidences are provided for novel structural features of human NATs that are absent in bacterial enzymes, including an insertion that produces a significant difference in the structure of the carboxyl terminus of the eukaryotic enzymes. A complete picture of the distinct substrate selectivity of human NAT1 and NAT2, as well as key features of the naturally occurring variants of NAT1 and NAT2 is provided Homo sapiens

Protein Variants

Protein Variants Comment Organism
F125S high resolution crystal structure of mutant F125S is generated Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-bromoacetanilide irreversible inhibitor Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Homo sapiens

Synonyms

Synonyms Comment Organism
arylamine N-acetyltransferase 1
-
 Homo sapiens
NAT1
-
 Homo sapiens
NAT2
-
 Homo sapiens