Protein Variants | Comment | Organism |
---|---|---|
M209T | replacement of residue M209 with the corresponding residue from Mycobacterium tuberculosis enzyme. Activities against several substrates similar to wild-type, with similar overall trends | Mycobacterium marinum |
additional information | replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis and vice versa | Mycobacterium tuberculosis |
additional information | replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis and vice versa | Mycobacterium marinum |
Y71F | replacement of residue Y71 with the corresponding residue from Mycobacterium tuberculosis enzyme. Activities against several substrates similar to wild-type, with similar overall trends | Mycobacterium marinum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
acetyl-CoA | chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C | Mycobacterium tuberculosis | |
0.07 | - |
acetyl-CoA | chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C | Mycobacterium marinum | |
0.158 | - |
acetyl-CoA | wild-type, pH 8.0, 28°C | Mycobacterium marinum | |
0.181 | - |
acetyl-CoA | chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C | Mycobacterium tuberculosis | |
0.181 | - |
acetyl-CoA | chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C | Mycobacterium marinum | |
1 | - |
5-aminosalicylic acid | wild-type, pH 8.0, 28°C | Mycobacterium tuberculosis | |
1.2 | - |
5-aminosalicylic acid | chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C | Mycobacterium tuberculosis | |
1.2 | - |
5-aminosalicylic acid | chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C | Mycobacterium marinum | |
1.2 | - |
5-aminosalicylic acid | chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C | Mycobacterium tuberculosis | |
1.2 | - |
5-aminosalicylic acid | chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C | Mycobacterium marinum | |
1.7 | - |
5-aminosalicylic acid | wild-type, pH 8.0, 28°C | Mycobacterium marinum | |
1.8 | - |
4-anisidine | chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C | Mycobacterium tuberculosis | |
1.8 | - |
4-anisidine | chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C | Mycobacterium marinum | |
2.3 | - |
4-anisidine | chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C | Mycobacterium tuberculosis | |
2.3 | - |
4-anisidine | chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C | Mycobacterium marinum | |
3.7 | - |
4-anisidine | wild-type, pH 8.0, 28°C | Mycobacterium marinum | |
4.7 | - |
isoniazid | chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C | Mycobacterium tuberculosis | |
4.7 | - |
isoniazid | chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C | Mycobacterium marinum | |
6.1 | - |
isoniazid | chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C | Mycobacterium tuberculosis | |
6.1 | - |
isoniazid | chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C | Mycobacterium marinum | |
6.2 | - |
isoniazid | wild-type, pH 8.0, 28°C | Mycobacterium marinum | |
560 | - |
acetyl-CoA | wild-type, pH 8.0, 28°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium marinum | - |
- |
- |
Mycobacterium tuberculosis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 2-aminofluorene | - |
Mycobacterium tuberculosis | CoA + N-acetyl-2-aminofluorene | - |
? | |
acetyl-CoA + 2-aminofluorene | mutant Y71F, 128% of wild-type activity, mutant M209T, 62% of wild-type activity | Mycobacterium marinum | CoA + N-acetyl-2-aminofluorene | - |
? | |
acetyl-CoA + 3,4-dichloroaniline | - |
Mycobacterium tuberculosis | CoA + N-acetyl-3,4-dichloroaniline | - |
? | |
acetyl-CoA + 3,4-dichloroaniline | mutant Y71F, 194% of wild-type activity, mutant M209T, 88% of wild-type activity | Mycobacterium marinum | CoA + N-acetyl-3,4-dichloroaniline | - |
? | |
acetyl-CoA + 4-aminosalicylic acid | - |
Mycobacterium tuberculosis | CoA + N-acetyl-4-aminosalicylic acid | - |
? | |
acetyl-CoA + 4-aminosalicylic acid | mutant Y71F, 17% of wild-type activity, mutant M209T, 128% of wild-type activity | Mycobacterium marinum | CoA + N-acetyl-4-aminosalicylic acid | - |
? | |
acetyl-CoA + 4-anisidine | - |
Mycobacterium tuberculosis | CoA + N-acetyl-4-anisidine | - |
? | |
acetyl-CoA + 4-anisidine | mutant Y71F, 860% of wild-type activity, mutant M209T, 673% of wild-type activity | Mycobacterium marinum | CoA + N-acetyl-4-anisidine | - |
? | |
acetyl-CoA + 4-chloroaniline | - |
Mycobacterium tuberculosis | CoA + N-acetyl-4-chloroaniline | - |
? | |
acetyl-CoA + 4-chloroaniline | mutant Y71F, 174% of wild-type activity, mutant M209T, 122% of wild-type activity | Mycobacterium marinum | CoA + N-acetyl-4-chloroaniline | - |
? | |
acetyl-CoA + 4-iodoaniline | - |
Mycobacterium tuberculosis | CoA + N-acetyl-4-iodoaniline | - |
? | |
acetyl-CoA + 4-iodoaniline | mutant Y71F, 196% of wild-type activity, mutant M209T, 83% of wild-type activity | Mycobacterium marinum | CoA + N-acetyl-4-iodoaniline | - |
? | |
acetyl-CoA + 5-aminosalicylic acid | - |
Mycobacterium tuberculosis | CoA + N-acetyl-5-aminosalicylic acid | - |
? | |
acetyl-CoA + 5-aminosalicylic acid | mutant Y71F, 147% of wild-type activity, mutant M209T, 21% of wild-type activity | Mycobacterium marinum | CoA + N-acetyl-5-aminosalicylic acid | - |
? | |
acetyl-CoA + isoniazid | - |
Mycobacterium tuberculosis | CoA + N-acetylisoniazid | - |
? | |
acetyl-CoA + isoniazid | mutant Y71F, 102% of wild-type activity, mutant M209T, 229% of wild-type activity | Mycobacterium marinum | CoA + N-acetylisoniazid | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
5-aminosalicylic acid | wild-type, pH 8.0, 28°C | Mycobacterium tuberculosis | |
0.1 | - |
isoniazid | chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C | Mycobacterium tuberculosis | |
0.1 | - |
isoniazid | chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C | Mycobacterium marinum | |
0.3 | - |
isoniazid | chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C | Mycobacterium tuberculosis | |
0.3 | - |
isoniazid | chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C | Mycobacterium marinum | |
0.3 | - |
4-anisidine | chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C | Mycobacterium tuberculosis | |
0.3 | - |
4-anisidine | chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C | Mycobacterium marinum | |
0.6 | - |
4-anisidine | chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C | Mycobacterium tuberculosis | |
0.6 | - |
4-anisidine | chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C | Mycobacterium marinum | |
1.7 | - |
isoniazid | wild-type, pH 8.0, 28°C | Mycobacterium marinum | |
1.8 | - |
5-aminosalicylic acid | chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C | Mycobacterium tuberculosis | |
1.8 | - |
5-aminosalicylic acid | chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C | Mycobacterium marinum | |
1.8 | - |
5-aminosalicylic acid | chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C | Mycobacterium tuberculosis | |
1.8 | - |
5-aminosalicylic acid | chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C | Mycobacterium marinum | |
5.2 | - |
5-aminosalicylic acid | wild-type, pH 8.0, 28°C | Mycobacterium marinum | |
5.2 | - |
4-anisidine | wild-type, pH 8.0, 28°C | Mycobacterium marinum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
isoniazid | chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C | Mycobacterium tuberculosis | |
0.009 | - |
isoniazid | chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C | Mycobacterium marinum | |
0.018 | - |
5-aminosalicylic acid | wild-type, pH 8.0, 28°C | Mycobacterium tuberculosis | |
0.121 | - |
isoniazid | chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C | Mycobacterium tuberculosis | |
0.121 | - |
isoniazid | chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C | Mycobacterium marinum | |
0.166 | - |
4-anisidine | chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C | Mycobacterium tuberculosis | |
0.166 | - |
anisidine | chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C | Mycobacterium marinum | |
0.26 | - |
4-anisidine | chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C | Mycobacterium tuberculosis | |
0.26 | - |
anisidine | chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C | Mycobacterium marinum | |
0.274 | - |
isoniazid | wild-type, pH 8.0, 28°C | Mycobacterium marinum | |
1.405 | - |
anisidine | wild-type, pH 8.0, 28°C | Mycobacterium marinum | |
1.5 | - |
5-aminosalicylic acid | chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C | Mycobacterium tuberculosis | |
1.5 | - |
5-aminosalicylic acid | chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C | Mycobacterium marinum | |
1.5 | - |
5-aminosalicylic acid | chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C | Mycobacterium tuberculosis | |
1.5 | - |
5-aminosalicylic acid | chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C | Mycobacterium marinum | |
3.058 | - |
5-aminosalicylic acid | wild-type, pH 8.0, 28°C | Mycobacterium marinum |