Literature summary for 2.3.1.5 extracted from

Cocaign, A.; Kubiak, X.; Xu, X.; Garnier, G.; Li de la Sierra-Gallay, I.; Chi-Bui, L.; Dairou, J.; Busi, F.; Abuhammad, A.; Haouz, A.; Dupret, J.M.; Herrmann, J.L.; Rodrigues-Lima, F.
Structural and functional characterization of an arylamine N-acetyltransferase from the pathogen Mycobacterium abscessus: differences from other mycobacterial isoforms and implications for selective inhibition (2014), Acta Crystallogr. Sect. D, 70, 3066-3079.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 cells	Mycobacteroides abscessus

Crystallization (Commentary)

Crystallization (Comment)	Organism
vapor diffusion method, using 15% (w/v) PEG 6000, 5% (v/v) glycerol at 18°C	Mycobacteroides abscessus

Inhibitors

Inhibitors	Comment	Organism	Structure
piperidinol	poor inhibition	Mycobacteroides abscessus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.146	-	2-Aminofluorene	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus
0.157	-	3,4-dichloroaniline	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus
0.393	-	hydralazine	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus
0.462	-	4-butoxyaniline	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus
0.662	-	5-Aminosalicylate	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus
1.386	-	4-iodoaniline	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus
3.827	-	4-Aminosalicylate	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus
6.754	-	isoniazid	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
31000	-	x * 31000, calculated from amino acid sequence	Mycobacteroides abscessus
37000	-	x * 37000, SDS-PAGE	Mycobacteroides abscessus

Organism

Organism	UniProt	Comment	Textmining
Mycobacteroides abscessus	-	-	-
Mycobacteroides abscessus CIP 104536T	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Mycobacteroides abscessus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + 2-aminofluorene	-	Mycobacteroides abscessus	CoA + N-acetyl-2-aminofluorene	-	?
acetyl-CoA + 2-aminofluorene	-	Mycobacteroides abscessus CIP 104536T	CoA + N-acetyl-2-aminofluorene	-	?
acetyl-CoA + 3,4-dichloroaniline	-	Mycobacteroides abscessus	CoA + N-acetyl-3,4-dichloroaniline	-	?
acetyl-CoA + 3,4-dichloroaniline	-	Mycobacteroides abscessus CIP 104536T	CoA + N-acetyl-3,4-dichloroaniline	-	?
acetyl-CoA + 4-aminosalicylate	-	Mycobacteroides abscessus	CoA + N-acetyl-4-aminosalicylate	-	?
acetyl-CoA + 4-butoxyaniline	-	Mycobacteroides abscessus	CoA + N-acetyl-4-butoxyaniline	-	?
acetyl-CoA + 4-iodoaniline	-	Mycobacteroides abscessus	CoA + N-acetyl-4-iodoaniline	-	?
acetyl-CoA + 4-iodoaniline	-	Mycobacteroides abscessus CIP 104536T	CoA + N-acetyl-4-iodoaniline	-	?
acetyl-CoA + 5-aminosalicylate	-	Mycobacteroides abscessus	CoA + N-acetyl-5-aminosalicylate	-	?
acetyl-CoA + hydralazine	-	Mycobacteroides abscessus	CoA + N-acetyl-hydralazine	-	?
acetyl-CoA + isoniazid	-	Mycobacteroides abscessus	CoA + N-acetyl-isoniazid	-	?
acetyl-CoA + isoniazid	-	Mycobacteroides abscessus CIP 104536T	CoA + N-acetyl-isoniazid	-	?
additional information	no activity with 4-aminobenzoic acid, ambroxol, trimethoprim, sulfamethazine, sulfamethoxazole, sulfadiazine, and sulfapyridine	Mycobacteroides abscessus	?	-	?
additional information	no activity with 4-aminobenzoic acid, ambroxol, trimethoprim, sulfamethazine, sulfamethoxazole, sulfadiazine, and sulfapyridine	Mycobacteroides abscessus CIP 104536T	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 37000, SDS-PAGE	Mycobacteroides abscessus
?	x * 31000, calculated from amino acid sequence	Mycobacteroides abscessus

Synonyms

Synonyms	Comment	Organism
(MYCAB)NAT1	-	Mycobacteroides abscessus
NAT	-	Mycobacteroides abscessus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1	-	4-Aminosalicylate	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus
8	-	3,4-dichloroaniline	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus
9	-	isoniazid	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus
47	-	5-Aminosalicylate	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus
71	-	4-iodoaniline	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus
112	-	4-butoxyaniline	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus
168	-	hydralazine	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus
199	-	2-Aminofluorene	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.252	-	4-Aminosalicylate	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus
0.596	-	4-iodoaniline	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus
1.317	-	isoniazid	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus
71.3	-	5-Aminosalicylate	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus
244.4	-	4-butoxyaniline	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus
434.5	-	hydralazine	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus
525.6	-	3,4-dichloroaniline	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus
1363	-	2-Aminofluorene	apparent value, at pH 7.5 and 37°C	Mycobacteroides abscessus

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Release 2023.1 (January 2023)