Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mesorhizobium loti |
Crystallization (Comment) | Organism |
---|---|
in complex with CoA, sitting drop vapor diffusion method, using 20% (w/v) PEG 4000, 0.6 M NaCl, 0.1 M MES buffer pH 6.5 | Mesorhizobium loti |
Protein Variants | Comment | Organism |
---|---|---|
F42W | the mutant displays enzymatic properties similar to those of the wild type enzyme | Mesorhizobium loti |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesorhizobium loti | Q98D42 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Mesorhizobium loti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 4-aminosalicylate | - |
Mesorhizobium loti | CoA + N-acetyl-4-aminosalicylate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAT1 | - |
Mesorhizobium loti |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9 | - |
4-Aminosalicylate | at pH 7.5 and 25°C | Mesorhizobium loti | |
15 | - |
acetyl-CoA | at pH 7.5 and 25°C | Mesorhizobium loti |