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Literature summary for 2.3.1.50 extracted from

  • Lynch, D.V.; Fairfield, S.R.
    Sphingolipid long-chain base synthesis in plants. Characterization of serine palmitoyltransferase activity in squash fruit microsomes (1993), Plant Physiol., 103, 1421-1429.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.50

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol required for enzyme activity Cucurbita pepo
EDTA required for enzyme activity Cucurbita pepo

Inhibitors

Inhibitors Comment Organism Structure
beta-chloro-L-alanine mechanism-based inhibition Cucurbita pepo
L-Cycloserine mechanism-based inhibition Cucurbita pepo

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8
-
 L-serine
-
 Cucurbita pepo

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Cucurbita pepo 5783
-
microsome
-
 Cucurbita pepo
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
palmitoyl-CoA + L-serine Cucurbita pepo
-
 CoA + 3-dehydro-D-sphinganine + CO2
-
 ir

Organism

Organism UniProt Comment Textmining
Cucurbita pepo
-
 cv. early profilic straightneck
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit age-dependent amount of enzyme activity Cucurbita pepo
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.057 0.084
-
 Cucurbita pepo

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-CoA + L-serine
-
 Cucurbita pepo CoA + 3-dehydro-D-sphinganine + CO2
-
 ir
palmitoyl-CoA + L-serine palmitoyl-CoA is the preferred substrate Cucurbita pepo CoA + 3-dehydro-D-sphinganine + CO2
-
 ir
palmitoyl-CoA + L-serine optimal palmitoyl-CoA concentration is 0.2 mM Cucurbita pepo CoA + 3-dehydro-D-sphinganine + CO2
-
 ir
palmitoyl-CoA + L-serine palmitoyl-CoA is used in preference to other saturated or unsaturated acyl-CoA substrates Cucurbita pepo CoA + 3-dehydro-D-sphinganine + CO2
-
 ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
36 40
-
 Cucurbita pepo

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Cucurbita pepo

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate stimulates Cucurbita pepo