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Literature summary for 2.3.1.50 extracted from
- Cloning and initial characterization of a new subunit for mammalian serine-palmitoyltransferase (2006), J. Biol. Chem., 281, 37275-37281.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of subunit SPTLC3 in HEK-293 cells, leading to an 2-3fold increased SPT activity, expression of subunit SPTLC3 in Hep-G2 cells and human trophoblast cells, quantitative expression analysis of SPTLC3 | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum membrane | the enzyme is a transmembrane protein | Homo sapiens | 5789 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | the expression of two SPT isoforms could be a cellular mechanism to adjust SPT activity to tissue-specific requirements of sphingolipid synthesis | ? | - |
? |  |
| palmitoyl-CoA + L-serine | Homo sapiens | the enzyme catalyses the rate limiting step for the de novo synthesis of sphingolipids | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HEK-293 cell | very low SPTLC3 content | Homo sapiens | - |
| placenta | high SPTLC3 content | Homo sapiens | - |
| trophoblast | high SPTLC3 content | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the expression of two SPT isoforms could be a cellular mechanism to adjust SPT activity to tissue-specific requirements of sphingolipid synthesis | Homo sapiens | ? | - |
? | |
| palmitoyl-CoA + L-serine | - |
Homo sapiens | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
| palmitoyl-CoA + L-serine | the enzyme catalyses the rate limiting step for the de novo synthesis of sphingolipids | Homo sapiens | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | the enzyme is composed of three different types of subunits SPTLC1, SPTLC2, and SPTLC3 | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| serine-palmitoyltransferase | - |
Homo sapiens |
| SPT | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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