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Literature summary for 2.3.1.51 extracted from

  • Okuyama, H.; Wakil, S.J.
    Positional specificities of acyl coenzyme A: glycerophosphate and acyl coenzyme A: monoacylglycerophosphate acyltransferases in Escherichia coli (1973), J. Biol. Chem., 248, 5197-5205.
    View publication on PubMed

General Stability

General Stability Organism
slow freezing inactivates Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
MgCl2 at high concentrations Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Escherichia coli 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no Mg2+-requirement Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
B
-
Escherichia coli B / ATCC 11303
-
B
-

Storage Stability

Storage Stability Organism
-15°C to 4°C, particulate preparation, fast-frozen in liquid N2, unstable Escherichia coli
-15°C, less than a day Escherichia coli
-80°C, particulate preparation, fast-frozen in liquid N2, 3 months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
Escherichia coli CoA + 1,2-diacyl-sn-glycerol 3-phosphate i.e. phosphatidic acid ?
acyl-CoA + 2-acyl-sn-glycerol 3-phosphate the enzyme does not acylate 2-acyl-sn-glycerol 3-phosphate Escherichia coli CoA + ?
-
?
additional information effect of acyl-CoA concentrations on enzyme reaction Escherichia coli ?
-
?
additional information 1-acyl-sn-glycerol 3-phosphate is readily acylated by unsaturated and saturated acyl-CoAs Escherichia coli ?
-
?
additional information effect of acyl-CoA concentrations on enzyme reaction Escherichia coli B / ATCC 11303 ?
-
?
additional information 1-acyl-sn-glycerol 3-phosphate is readily acylated by unsaturated and saturated acyl-CoAs Escherichia coli B / ATCC 11303 ?
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate twice as good as palmitoyl-CoA Escherichia coli CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
Escherichia coli CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli
7.5 8
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6.6 8.6 about half-maximal activity at pH 6.6 and 8.6 Escherichia coli