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Literature summary for 2.3.1.51 extracted from
- sn-1-Acylglycerol-3-phosphate acyltransferase of Escherichia coli causes insertion of cis-11 eicosenoic acid into the sn-2 position of transgenic rapeseed oil (1998), Mol. Breed., 4, 39-46.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| modification of the gene of Escherichia coli which encodes the enzyme by inserting an endoplasmic reticulum retrieval signal to its 3'end and introduccion into rapeseed plants under the control of a napin promotor | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica napus | - |
- |
- |
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| capryl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-acyl-2-capryl-sn-glycerol 3-phosphate | - |
? |  |
| capryl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Brassica napus | CoA + 1-acyl-2-capryl-sn-glycerol 3-phosphate | - |
? | |
| cis-11-eicosenoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-acyl-2-cis-11 eicosenoyl-sn-glycerol 3-phosphate | - |
? | |
| cis-11-eicosenoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Brassica napus | CoA + 1-acyl-2-cis-11 eicosenoyl-sn-glycerol 3-phosphate | - |
? | |
| erucoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-acyl-2-erucoyl-sn-glycerol 3-phosphate | - |
? | |
| erucoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Brassica napus | CoA + 1-acyl-2-erucoyl-sn-glycerol 3-phosphate | - |
? | |
| lauroyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-acyl-2-lauroyl-sn-glycerol 3-phosphate | - |
? | |
| lauroyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Brassica napus | CoA + 1-acyl-2-lauroyl-sn-glycerol 3-phosphate | - |
? | |
| additional information | an introduced chimeric enzyme gene of E. coli alters the stereochemical composition of transgenic HEAR oil by preferentially directing cis-11 eicosenoic acid to its sn-2 position | Escherichia coli | ? | - |
? | |
| oleoyl-ACP + sn-1-oleoylglycerol 3-phosphate | microsomal fractions from developing, transgenic seeds but not of untransformed plants | Escherichia coli | ACP + sn-1,2-dioleoylglycerol 3-phosphate | - |
? | |
| oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate | - |
? | |
| oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Brassica napus | CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate | - |
? | |
| palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
| palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Brassica napus | CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
| 30 | - |
assay at | Brassica napus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
assay at | Escherichia coli |
| 9 | - |
assay at | Brassica napus |
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