Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.51 extracted from

  • Bourgis, F.; Kader, J.C.; Barret, P.; Renard, M.; Robinson, D.; Robinson, C.; Delseny, M.; Roscoe, T.J.
    A plastidial lysophosphatidic acid acyltransferase from oilseed rape (1999), Plant Physiol., 120, 913-922.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.51

Cloned(Commentary)

Cloned (Comment) Organism
isolation of a cDNA encoding an enzyme by functional complementation of the Escherichia coli mutant plsC with an immature embryo cDNA library of oilseed rape. Transformation of the acyltransferase-deficient Escherichia coli strain JC201 with the cDNA sequence BAT2 alleviates the temperature-sensitive phenotype of the plsC mutant and confers a palmitoyl-CoA-preferring enzyme activity to membrane fractions. Mapping of the BAT2 genes and sequence analysis of the BAT2 cDNA clone Brassica napus

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast mature protein of 32000 Da processed from the precursor protein of 38000 Da Brassica napus 9507
-
membrane
-
 Brassica napus 16020
-
plastid
-
 Brassica napus 9536
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 mature protein, processing of a BAT2 precursor protein, chloroplast-import experiments Brassica napus
38000
-
 predicted, protein encoded by the BAT2 cDNA Brassica napus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Brassica napus the enzyme controls phosphatidic acid biosynthesis in the plastid ?
-
 ?

Organism

Organism UniProt Comment Textmining
Brassica napus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information the BAT2 gene is expressed in a ubiquitous manner in all tissues, including nonphotosynthetic tissues where plastids are present Brassica napus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
 Brassica napus CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
-
 ?
additional information the enzyme controls phosphatidic acid biosynthesis in the plastid Brassica napus ?
-
 ?
oleoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate assayed with BAT2 protein Brassica napus CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
 ?
palmitoyl-ACP + 1-acyl-sn-glycerol 3-phosphate
-
 Brassica napus ACP + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
 ?
palmitoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate assayed with BAT2 protein, palmitoyl-CoA is the preferred substrate Brassica napus CoA + 1-oleoyl-2-palmitoyl-sn-glycerol 3-phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Brassica napus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Brassica napus