Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli BL21 (DE3), pCMV-SPORT6 vector, overexpressed in Saccharomyces cerevisiae, pYES2 vector | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E260K | site-directed mutagenesis, mutation found in Chanarin-Dorfman syndrome patients, mutation without significant effect on acyltransferase activity | Homo sapiens |
Q130P | site-directed mutagenesis, mutation found in Chanarin-Dorfman syndrome patients, mutation without significant effect on acyltransferase activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
lysophosphatidylcholine | inhibits 68% of the enzyme activity at 30 microM | Homo sapiens | |
Phospholipids | activity inhibited by high concentrations of | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
palmitoyl-CoA | stearoyl-CoA as an acyl donor reveals only 33% of the activity observed under the standard assay conditions | Homo sapiens | |
0.014 | - |
oleoyl-CoA | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | white adipose tissue of mice, precise role of cgi-58 protein in the cytoplasm unknown | Homo sapiens | 5829 | - |
lipid droplet | white adipose tissue of mice | Homo sapiens | 5811 | - |
membrane | more than 60% of total activity associated with membranes in white adipose tissue | Homo sapiens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
45 kDa protein in Western Blot and mass spectrometry analysis, subcellular fractions of white adipose tissue | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | Homo sapiens | - |
CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration and SDS-PAGE, recombinant protein, mutant and wild-type | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
white adipose tissue | physiological significance analyzed in white adipose tissue of mice, soluble lysophosphatidic acid acyltransferase activity, existence of an alternate cytosolic phosphatidic acid biosynthetic pathway | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Homo sapiens | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | substrate preference analyzed, assays performed with different acyl donors | Homo sapiens | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
oleoyl-CoA + 1-oleoyl-sn-glycerol-3-phosphate | substrate preference higher for oleoyl-CoA than for palmitoyl-CoA and stearoyl-CoA | Homo sapiens | CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Homo sapiens | CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cgi-58 protein | - |
Homo sapiens |
lysophosphatidic acid ayltransferase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity assay at | Homo sapiens |