Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli, overexpression in Saccharomyces cerevisiae, expression plasmid pPS189 | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | Saccharomyces cerevisiae | - |
CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
BY4741 and deletion mutant strain of ICT1 protein in BY4741-background | - |
Purification (Comment) | Organism |
---|---|
gel filtration and SDS-PAGE, recombinant protein | Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Saccharomyces cerevisiae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
absence of transmembrane domains, presence of hydrolase/acyltransferase domain with a distinct lipid-binding motif and a lysophospholipase domain, reduced phosphatidic acid in mutants suggests a role in phosphatidic acid biosynthesis, overexpression shows increase in phosphatidic acid and other phospholipids on organic solvent exposure, recombinant protein acylates lysophosphatidic acid, substrate preference analyzed, nearly no activity with lysophospholipids such as lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE), and lysophosphatidylserine (LPS) | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Saccharomyces cerevisiae | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
oleoyl-CoA + 1-oleoyl-sn-glycerol-3-phosphate | substrate preference higher for oleoyl-CoA than for palmitoyl-CoA and stearoyl-CoA | Saccharomyces cerevisiae | CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | substrate preference lower than for oleoyl-CoA | Saccharomyces cerevisiae | CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ICT1 protein | - |
Saccharomyces cerevisiae |
lysophosphatidic acid ayltransferase | - |
Saccharomyces cerevisiae |
oleoyl-CoA lysophosphatidic acid acyltransferase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity assay at | Saccharomyces cerevisiae |