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Literature summary for 2.3.1.51 extracted from

  • Agarwal, A.K.; Sukumaran, S.; Bartz, R.; Barnes, R.I.; Garg, A.
    Functional characterization of human 1-acylglycerol-3-phosphate-O-acyltransferase isoform 9: cloning, tissue distribution, gene structure, and enzymatic activity (2007), J. Endocrinol., 193, 445-457.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli, plasmids pCR2.1, pDrive, pcDNA 3.1(C)neo, construction of the wild-type fusion protein with GFP, overexpression in CHO-cells, coexpression in CHO-cells with AGPAT1 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum same domain as AGPAT1 Homo sapiens 5783
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate Homo sapiens activity and expression pattern of AGPAT9 in lung and spleen implicates role in the biosynthesis of phospholipids and triglycerides in these tissues CoA + 1,2-diacyl-sn-glycerol 3-phosphate
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?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8NF37
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Source Tissue

Source Tissue Comment Organism Textmining
adipose tissue expressed in Homo sapiens
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leukocyte expressed in Homo sapiens
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lung high expression in Homo sapiens
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placenta expressed in Homo sapiens
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spleen high expression in Homo sapiens
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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overexpression shows 1-acyl-sn-glycero-3-phosphate acyltransferase activity but no lysophosphatidylcholine acyltransferase activity, acyl-CoA specificity and specificity of lysophosphatidic acids (LPAs) listed, substitution of asparagine with proline in the NHX4D motif and arginine with cysteine in the EGTR motif, mutant retains enzyme activity, model proposed in which a hydrophobic pocket in 1-acyl-sn-glycero-3-phosphate acyltransferase 9 accommodates fatty acyl chains of both substrates in proper orientation, whereas the HX4D motif participates in catalysis Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate activity and expression pattern of AGPAT9 in lung and spleen implicates role in the biosynthesis of phospholipids and triglycerides in these tissues Homo sapiens CoA + 1,2-diacyl-sn-glycerol 3-phosphate
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?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate cloning and characterization of isoform AGPAT9 Homo sapiens CoA + 1,2-diacyl-sn-glycerol 3-phosphate
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?

Synonyms

Synonyms Comment Organism
1-acyl-sn-glycero-3-phosphate acyltransferase 9
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Homo sapiens
AGPAT9
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Homo sapiens