Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli, plasmids pCR2.1, pDrive, pcDNA 3.1(C)neo, construction of the wild-type fusion protein with GFP, overexpression in CHO-cells, coexpression in CHO-cells with AGPAT1 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | same domain as AGPAT1 | Homo sapiens | 5783 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | Homo sapiens | activity and expression pattern of AGPAT9 in lung and spleen implicates role in the biosynthesis of phospholipids and triglycerides in these tissues | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8NF37 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adipose tissue | expressed in | Homo sapiens | - |
leukocyte | expressed in | Homo sapiens | - |
lung | high expression in | Homo sapiens | - |
placenta | expressed in | Homo sapiens | - |
spleen | high expression in | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
overexpression shows 1-acyl-sn-glycero-3-phosphate acyltransferase activity but no lysophosphatidylcholine acyltransferase activity, acyl-CoA specificity and specificity of lysophosphatidic acids (LPAs) listed, substitution of asparagine with proline in the NHX4D motif and arginine with cysteine in the EGTR motif, mutant retains enzyme activity, model proposed in which a hydrophobic pocket in 1-acyl-sn-glycero-3-phosphate acyltransferase 9 accommodates fatty acyl chains of both substrates in proper orientation, whereas the HX4D motif participates in catalysis | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | activity and expression pattern of AGPAT9 in lung and spleen implicates role in the biosynthesis of phospholipids and triglycerides in these tissues | Homo sapiens | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | cloning and characterization of isoform AGPAT9 | Homo sapiens | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1-acyl-sn-glycero-3-phosphate acyltransferase 9 | - |
Homo sapiens |
AGPAT9 | - |
Homo sapiens |