Application | Comment | Organism |
---|---|---|
medicine | expression of isoform CGI-58 in fibroblasts from humans with Chanarin-Dorfman increases the incorporation of fatty acids released from the lipolysis of stored triacylglycerols into phospholipids | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mus musculus |
expression of active His6-tagged CGI-58 in Escherichia coli | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | recombinant expression of murine CGI-58 in fibroblasts from humans with Chanarin-Dorfman syndrome increases the incorporation of radiolabeled fatty acids released from the lipolysis of stored triacylglycerols into phospholipids | Mus musculus |
additional information | recombinant expression of murine CGI-58 in fibroblasts from humans with Chanarin-Dorfman syndrome increases the incorporation of radiolabeled fatty acids released from the lipolysis of stored triacylglycerols into phospholipids | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 1 microM, 60% inhibition. Addition of EDTA restores activity | Mus musculus | |
MgCl2 | 1 microM, 35% inhibition. Addition of EDTA restores activity | Mus musculus | |
Triton X-100 | 0.4 mM, inhibition | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0048 | - |
oleoyl-CoA | pH 7.5, 30°C | Mus musculus | |
0.018 | - |
1-oleoyl-sn-glycerol 3-phosphate | pH 7.5, 30°C | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | Mus musculus | i.e. lysophosphatidic acid | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | i.e. phosphatidic acid | ? | |
additional information | Mus musculus | CGI-58 is a CoA-dependent lysophosphatidic acid acyltransferase that channels fatty acids released from the hydrolysis of stored triacylglycerols into phospholipids, overview | ? | - |
? | |
additional information | Homo sapiens | CGI-58 is a CoA-dependent lysophosphatidic acid acyltransferase that channels fatty acids released from the hydrolysis of stored triacylglycerols into phospholipids, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Mus musculus | Q9DBL9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | i.e. lysophosphatidic acid | Mus musculus | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | i.e. phosphatidic acid | ? | |
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | i.e. lysophosphatidic acid, the recombinant CGI-58 shows preference for arachidonoyl-CoA and oleoyl-CoA, and slight preference for 1-oleoyl lysophosphatidic acid over 1-palmitoyl, 1-stearoyl, or 1-arachidonoyl lysophosphatidic acid | Mus musculus | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | i.e. phosphatidic acid | ? | |
arachidonoyl-CoA + 1-arachidonoyl-2-lyso-sn-phosphatidylcholine | - |
Mus musculus | CoA + 1,2-diarachidonoyl-sn-phosphatidylcholine | - |
? | |
arachidonoyl-CoA + 1-arachidonoyl-sn-glycerol 3-phosphate | slight preference for 1-oleoyl lysophosphatidic acid over 1-palmitoyl, 1-stearoyl, or 1-arachidonoyl lysophosphatidic acid | Mus musculus | CoA + 1,2-diarachidonoyl-sn-glycerol 3-phosphate | - |
? | |
arachidonoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate | and oleoyl-CoA, preferred substrates | Mus musculus | CoA + 1-oleoyl-2-arachidonoyl-sn-glycerol 3-phosphate | - |
? | |
arachidonoyl-CoA + 1-oleoyl-sn-lysophosphatidylcholine | - |
Mus musculus | CoA + 1-oleoyl-2-arachidonoylphosphatidylcholine | - |
? | |
arachidonoyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate | slight preference for 1-oleoyl lysophosphatidic acid over 1-palmitoyl, 1-stearoyl, or 1-arachidonoyl lysophosphatidic acid | Mus musculus | CoA + 1-palmitoyl-2-arachidonoyl-sn-glycerol 3-phosphate | - |
? | |
arachidonoyl-CoA + 1-palmitoyl-sn-lysophosphatidylcholine | - |
Mus musculus | CoA + 1-palmitoyl-2-arachidonoylphosphatidylcholine | - |
? | |
arachidonoyl-CoA + 1-stearoyl-sn-glycerol 3-phosphate | slight preference for 1-oleoyl lysophosphatidic acid over 1-palmitoyl, 1-stearoyl, or 1-arachidonoyl lysophosphatidic acid | Mus musculus | CoA + 1-oleoyl-2-stearoyl-sn-glycerol 3-phosphate | - |
? | |
arachidonoyl-CoA + 1-stearoyl-sn-lysophosphatidylcholine | - |
Mus musculus | CoA + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphocholine | - |
? | |
additional information | CGI-58 is a CoA-dependent lysophosphatidic acid acyltransferase that channels fatty acids released from the hydrolysis of stored triacylglycerols into phospholipids, overview | Mus musculus | ? | - |
? | |
additional information | CGI-58 is a CoA-dependent lysophosphatidic acid acyltransferase that channels fatty acids released from the hydrolysis of stored triacylglycerols into phospholipids, overview | Homo sapiens | ? | - |
? | |
additional information | the CGI-58 carboxyl terminus includes a highly conserved consensus sequence, HXXXXD, required for acyltransferase activity | Homo sapiens | ? | - |
? | |
additional information | the recombinant CoA-dependent His-tagged CGI-58 is active with lysophosphatidic acid, but not with other lysophospholipid or neutral glycerolipid acceptors | Mus musculus | ? | - |
? | |
oleoyl-CoA + 1-arachidonoyl-sn-lysophosphatidylcholine | - |
Mus musculus | CoA + 1-arachidonoyl-2-oleoyl-sn-glycero-3-phosphocholine | - |
? | |
oleoyl-CoA + 1-oleoyl-2-lysophosphatidylcholine | - |
Mus musculus | CoA + 1,2-dioleoyl-sn-glycero-3-phosphocholine | - |
? | |
oleoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate | and arachidonoyl-CoA, preferred substrates. Slight preference for 1-oleoyl lysophosphatidic acid over 1-palmitoyl, 1-stearoyl, or 1-arachidonoyl lysophosphatidic acid | Mus musculus | CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate | - |
? | |
oleoyl-CoA + 1-palmitoyl-sn-lysophosphatidylcholine | - |
Mus musculus | CoA + 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine | - |
? | |
oleoyl-CoA + 1-stearoyl-sn-lysophosphatidylcholine | - |
Mus musculus | CoA + 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the CGI-58 carboxyl terminus includes a highly conserved consensus sequence, HXXXXD, required for acyltransferase activity | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
ABHD5 | - |
Mus musculus |
CGFI-58 | - |
Mus musculus |
CGI-58/ABHD5 | - |
Mus musculus |
CGI-58/ABHD5 | - |
Homo sapiens |
coenzyme A-dependent lysophosphatidic acid | - |
Mus musculus |
coenzyme A-dependent lysophosphatidic acid | - |
Homo sapiens |
More | the enzyme belongs to the alpha/beta-hydrolase family | Mus musculus |
More | the enzyme belongs to the alpha/beta-hydrolase family | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
47 | - |
10 min, 60% loss of activity | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | mutations in human CGI-58/ABHD5 cause Chanarin-Dorfman syndrome, characterized by excessive storage of triacylglycerol in tissues | Homo sapiens |
physiological function | expression of isoform CGI-58 in fibroblasts from humans with Chanarin-Dorfman increases the incorporation of fatty acids released from the lipolysis of stored triacylglycerols into phospholipids | Mus musculus |