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Literature summary for 2.3.1.54 extracted from

  • Conradt, H.; Hohmann-Berger, M.; Hohmann, H.P.; Blaschkowski, H.P.; Knappe, J.
    Pyruvate formate-lyase (inactive form) and pyruvate formate-lyase activating enzyme of Escherichia coli: isolation and structural properties (1984), Arch. Biochem. Biophys., 228, 133-142.
    View publication on PubMed
Search for more literature for 2.3.1.54

Activating Compound

Activating Compound Comment Organism Structure
S-adenosylmethionine
-
 Escherichia coli
S-adenosylmethionine
-
 Streptococcus mutans

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli SP 264 contains plasmid p29with pfl gene, identification of pfl gene product as a 80kDa polypeptide Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
iodoacetate
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 identification of pfl gene product Escherichia coli
85000
-
 2 * 85000, homodimer, SDS-PAGE Escherichia coli
85500
-
 2 * 85500, homodimer, SDS-PAGE Escherichia coli
151000
-
 sedimentation equilibrium Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate + CoA Streptococcus mutans
-
 acetyl-CoA + formate
-
 ?
pyruvate + CoA Escherichia coli key enzyme of the glucose fermentation route in anaerobically growing cells acetyl-CoA + formate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 K12
-
Escherichia coli
-
 ATCC 10798
-
Streptococcus mutans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
160
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, 10 mM MOPS-KOH, pH 7.0, 50 mM KCl, thiols, EDTA in 50% ethyleneglycol, proved stable for months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + CoA
-
 Escherichia coli acetyl-CoA + formate
-
 ?
pyruvate + CoA
-
 Streptococcus mutans acetyl-CoA + formate
-
 ?
pyruvate + CoA key enzyme of the glucose fermentation route in anaerobically growing cells Escherichia coli acetyl-CoA + formate
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Streptococcus mutans
dimer 2 * 85000, homodimer, SDS-PAGE Escherichia coli
dimer 2 * 85500, homodimer, SDS-PAGE Escherichia coli