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Literature summary for 2.3.1.54 extracted from
- Purification of pyruvate formate-lyase from Streptococcus mutans and its regulatory properties (1982), J. Bacteriol., 149, 1034-1040.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ferredoxin | - |
Escherichia coli | |
| Ferredoxin | - |
Clostridium butyricum | |
| Ferredoxin | methylviologen can replace ferredoxin | Streptococcus mutans | |
| oxamate | - |
Escherichia coli |  |
| oxamate | - |
Streptococcus mutans | |
| S-adenosylmethionine | - |
Escherichia coli | |
| S-adenosylmethionine | - |
Clostridium butyricum | |
| S-adenosylmethionine | - |
Streptococcus mutans | |
General Stability
| General Stability | Organism |
|---|---|
| unstable even when stored anaerobically | Streptococcus mutans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-Glyceraldehyde-3-phosphate | - |
Lactococcus lactis | |
| D-Glyceraldehyde-3-phosphate | - |
Streptococcus mutans | |
| dihydroxyacetone phosphate | - |
Lactococcus lactis | |
| dihydroxyacetone phosphate | - |
Streptococcus mutans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
2 * 90000, SDS-PAGE | Streptococcus mutans |
| 170000 | 180000 | gel filtration | Streptococcus mutans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + CoA | Escherichia coli | - |
acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | Lactococcus lactis | - |
acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | Enterococcus faecalis | - |
acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | Lactobacillus delbrueckii subsp. bulgaricus | - |
acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | Clostridium butyricum | - |
acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | Clostridium sp. | - |
acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | Streptococcus mutans | - |
acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | Streptococcus mutans JC2 | - |
acetyl-CoA + formate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium butyricum | - |
- |
- |
| Clostridium sp. | - |
- |
- |
| Enterococcus faecalis | - |
previously Streptococcus faecalis | - |
| Escherichia coli | - |
- |
- |
| Lactobacillus delbrueckii subsp. bulgaricus | - |
- |
- |
| Lactococcus lactis | - |
formerly designated Streptococcus lactis | - |
| Streptococcus mutans | - |
JC2 | - |
| Streptococcus mutans JC2 | - |
JC2 | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| immediately inactivated by exposure to the air | Streptococcus mutans |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
- |
Enterococcus faecalis |
- |
Streptococcus mutans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12 | - |
- |
Streptococcus mutans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 50 mM potassium phosphate buffer, containing 1 mM dithiothreitol, storage of the purified enzyme in an anaerobic glove box for 2 weeks reduces activity by 50% | Streptococcus mutans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + CoA | - |
Escherichia coli | acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | - |
Lactococcus lactis | acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | - |
Enterococcus faecalis | acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | - |
Lactobacillus delbrueckii subsp. bulgaricus | acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | - |
Clostridium butyricum | acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | - |
Clostridium sp. | acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | - |
Streptococcus mutans | acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | - |
Streptococcus mutans JC2 | acetyl-CoA + formate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Escherichia coli |
| dimer | 2 * 90000, SDS-PAGE | Streptococcus mutans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | 35 | activity is about 78 times of that at 22°C | Streptococcus mutans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Clostridium butyricum |
| 7.5 | - |
- |
Streptococcus mutans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.3 | 40% of activity maximum at pH 6.5, between pH 7.0-8.3 80% of maximum activity | Streptococcus mutans |
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