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Literature summary for 2.3.1.54 extracted from
- Computer-aided design of the stability of pyruvate formate-lyase from Escherichia coli by site-directed mutagenesis (2007), Biosci. Biotechnol. Biochem., 71, 746-753.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene pfl from strain JM109, DNA and amino acid sequence determination and analysis, expression of His-tagged wild-type and mutant enzymes in strain BL21(DE3) | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A273C | site-directed mutagenesis, the mutant is less thermostable than the wild-type enzyme | Escherichia coli |
| E336C | site-directed mutagenesis, the mutant is more thermostable than the wild-type enzyme, Tm is increased 3.7fold, half-life 1.8fold | Escherichia coli |
| E400I | site-directed mutagenesis, the mutant is more thermostable than the wild-type enzyme, Tm is increased 2.2fold, half-life 2.21fold | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state kinetics of wild-type and mutant enzymes | Escherichia coli | |
| 0.964 | - |
pyruvate | pH 7.6, 30°C, recombinant mutant A273C | Escherichia coli |  |
| 2.37 | - |
pyruvate | pH 7.6, 30°C, recombinant wild-type enzyme | Escherichia coli | |
| 3.55 | - |
pyruvate | pH 7.6, 30°C, recombinant mutant E400I | Escherichia coli | |
| 3.6 | - |
pyruvate | pH 7.6, 30°C, recombinant mutant E336C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA + pyruvate | Escherichia coli | - |
acetyl-CoA + formate | - |
? | |
| CoA + pyruvate | Escherichia coli JM109 | - |
acetyl-CoA + formate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli JM109 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from strain BL21(DE3) by nickel affinity chromatography to homogeneity | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA + pyruvate | - |
Escherichia coli | acetyl-CoA + formate | - |
? | |
| CoA + pyruvate | - |
Escherichia coli JM109 | acetyl-CoA + formate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | secondary structure, overview | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PFL | - |
Escherichia coli |
| pyruvate formate-lyase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | - |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
pH 7.5, half-lives of recombinant wild-type and mutant enzymes, overview | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | - |
Escherichia coli |
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