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Literature summary for 2.3.1.54 extracted from

  • Hemschemeier, A.; Jacobs, J.; Happe, T.
    Biochemical and physiological characterization of the pyruvate formate-lyase Pfl1 of Chlamydomonas reinhardtii, a typically bacterial enzyme in a eukaryotic alga (2008), Eukaryot. Cell, 7, 518-526.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3), pET9a expression vector, transformation into pyruvate formate-lyase-deficient Escherichia coli strain BL21, capacity for formate excretion shown by in vitro tests Chlamydomonas reinhardtii

Inhibitors

Inhibitors Comment Organism Structure
hypophosphite formate formation from pyruvate inhibited Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + pyruvate Chlamydomonas reinhardtii
-
 acetyl-CoA + formate
-
 ?

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii P37836 strain CC-125 and cell wall-less strain CC-277
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, Western Blot, heterologously expressed protein Chlamydomonas reinhardtii

Source Tissue

Source Tissue Comment Organism Textmining
cell culture formate accumulation inside anaerobically adapted Chlamydomonas reinhardtii CC-277 cells measured Chlamydomonas reinhardtii
-
culture medium formate accumulation in the medium of anaerobically adapted Chlamydomonas reinhardtii CC-277 cells measured Chlamydomonas reinhardtii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 ethanol-producing fermentative metabolism in Chlamydomonas reinhardtii proposed to be initiated by pyruvate formate-lyase, heterologous expression of Chlamydomonas Pfl1 protein in pyruvate formate-lyase-deficient strain of Escherichia coli, enzmye activity under anaerobic conditions regained, Pfl1 fermentation pathway of Chlamydomonas reinhardtii under physiological condition of sulfur depletion analyzed, formate accumulation measured Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + pyruvate
-
 Chlamydomonas reinhardtii acetyl-CoA + formate
-
 ?
CoA + pyruvate anaerobic metabolism and potential ethanol-producing pathways in Chlamydomonas reinhardtii analyzed Chlamydomonas reinhardtii acetyl-CoA + formate
-
 ?

Synonyms

Synonyms Comment Organism
Pfl1
-
 Chlamydomonas reinhardtii
pyruvate formate-lyase
-
 Chlamydomonas reinhardtii

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 activity assay at, wild-type and mutant protein Chlamydomonas reinhardtii