Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of the pfl deletion resulting in two different strains LL1164 and LL1170 with two different phenotypes, high lactate and low lactate, respectively. Of eight colonies picked, two have the LL1164 phenotype and six have the LL1170 phenotype. Strain LL1170 consumes more cellobiose, produces more acetate and ethanol and less lactate than strain LL1164. A pfor/pfl double deletion strain LL1178 consumes about 70% of the 5 g/L cellobiose initially present, which is about the same as its parent strain LL1141. It requires sodium acetate for growth, even in the presence of yeast extract. Lactate is the main product of fermentation | Thermoanaerobacterium saccharolyticum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + formate | Thermoanaerobacterium saccharolyticum | - |
CoA + pyruvate | - |
? | |
acetyl-CoA + formate | Thermoanaerobacterium saccharolyticum DSM 8691 | - |
CoA + pyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacterium saccharolyticum | I3VT07 | gene pfl or Tsac_0628 | - |
Thermoanaerobacterium saccharolyticum DSM 8691 | I3VT07 | gene pfl or Tsac_0628 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + formate | - |
Thermoanaerobacterium saccharolyticum | CoA + pyruvate | - |
? | |
acetyl-CoA + formate | - |
Thermoanaerobacterium saccharolyticum DSM 8691 | CoA + pyruvate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PFL | - |
Thermoanaerobacterium saccharolyticum |
pyruvate formate-lyase | - |
Thermoanaerobacterium saccharolyticum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Thermoanaerobacterium saccharolyticum |
Organism | Comment | Expression |
---|---|---|
Thermoanaerobacterium saccharolyticum | in all pyruvate ferredoxin oxidoreductase enzyme, gene pfor deletion strains, the expression levels of pyruvate formate-lyase genes are increased about sixfold compared with the parent strain. The strains show different growth dependent on the medium used | up |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme takes part in the metabolic pathway of pyruvate to ethanol in Thermoanaerobacterium saccharolyticum | Thermoanaerobacterium saccharolyticum |
physiological function | the physiological role of pyruvate formate-lyase is supplying C1 units for biosyntheses | Thermoanaerobacterium saccharolyticum |