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Literature summary for 2.3.1.57 extracted from

  • Chen, Y.; Vujcic, S.; Liang, P.; Diegelman, P.; Kramer, D.L.; Porter, C.W.
    Genomic identification and biochemical characterization of a second spermidine/spermine N1-acetyltransferase (2003), Biochem. J., 373, 661-667.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.57

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination of isozyme SSAT-2, localization of isozyme SSAT-2 on chromosome 17p13.1, expression of isozyme SSAT-2 in HEK-293 cells Homo sapiens
localization of isozyme SSAT-1 on chromosome Xp22 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + spermidine Homo sapiens
-
 CoA + N1-acetylspermidine
-
 ?
acetyl-CoA + spermine Homo sapiens
-
 CoA + N1-acetylspermine
-
 ?
additional information Homo sapiens isozyme SSAT-2 shows very low activity in intact wild-type cells, but is equally active to isozyme SSAT-1 in recombinantly transfected cells ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P21673 2 isozymes SSAT-1 and SSAT-2
-
Homo sapiens P21673 isozyme SSAT-1; 2 isozymes SSAT-1 and SSAT-2
-
Homo sapiens Q96F10 isozyme SSAT-2; 2 isozymes SSAT-1 and SSAT-2
-

Source Tissue

Source Tissue Comment Organism Textmining
small cell lung cancer cell isozyme SSAT-2 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens
0.00007
-
 recombinant HEK-293 cells expressing isozyme SSAT-2, substrate putrescine Homo sapiens
0.00008
-
 recombinant HEK-293 cells expressing isozyme SSAT-2, substrate N1-acetylspermine Homo sapiens
0.00009
-
 recombinant HEK-293 cells expressing isozyme SSAT-1, substrate putrescine Homo sapiens
0.0002
-
 recombinant HEK-293 cells expressing isozyme SSAT-1, substrate N1-acetylspermine Homo sapiens
0.00023
-
 recombinant HEK-293 cells expressing isozyme SSAT-2, substrate spermine Homo sapiens
0.00025
-
 recombinant HEK-293 cells expressing isozyme SSAT-2, substrate spermidine Homo sapiens
0.0004
-
 recombinant HEK-293 cells expressing isozyme SSAT-1, substrate spermine Homo sapiens
0.00068
-
 recombinant HEK-293 cells expressing isozyme SSAT-2, substrate norspermidine Homo sapiens
0.001
-
 recombinant HEK-293 cells expressing isozyme SSAT-1, substrate spermidine Homo sapiens
0.0011
-
 recombinant HEK-293 cells expressing isozyme SSAT-1, substrate norspermidine Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + N1-acetylspermine
-
 Homo sapiens CoA + N1,N12-diacetylspermine
-
 ?
acetyl-CoA + norspermidine
-
 Homo sapiens CoA + N1-acetylnorspermidine
-
 ?
acetyl-CoA + putrescine
-
 Homo sapiens CoA + N1-acetylputrescine
-
 ?
acetyl-CoA + spermidine
-
 Homo sapiens CoA + N1-acetylspermidine
-
 ?
acetyl-CoA + spermine
-
 Homo sapiens CoA + N1-acetylspermine
-
 ?
additional information isozyme SSAT-2 shows very low activity in intact wild-type cells, but is equally active to isozyme SSAT-1 in recombinantly transfected cells Homo sapiens ?
-
 ?
additional information substrate specificity of isozyme SSAT-1 in descending order: norspermidine equal spermidine, spermine, N1-acetylspermine, putrescine Homo sapiens ?
-
 ?
additional information substrate specificity of isozyme SSAT-2 in descending order: norspermidine, spermidine equal spermine, N1-acetylspermine equal putrescine Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
spermidine/spermine N1-acetyltransferase
-
 Homo sapiens
SSAT-1
-
 Homo sapiens
SSAT-2
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Homo sapiens