Application | Comment | Organism |
---|---|---|
analysis | colorimetric assay for monitoring enzyme SSAT1 activity in zebrafish using a 412-nm absorption spectrum in the presence of Ellman's reagent. The method is cost-effective and simple and also applicable to detect the cellular enzyme activity | Danio rerio |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.055 | - |
spermidine | pH 7.5, 25°C | Danio rerio | |
0.182 | - |
spermine | pH 7.5, 25°C | Danio rerio |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | Q6GQM2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + putrescine | poor substrate | Danio rerio | CoA + N1-acetylputrescine | - |
? | |
acetyl-CoA + spermidine | spermidine is preferred over spermine | Danio rerio | CoA + N1-acetyl-spermidine | - |
? | |
acetyl-CoA + spermine | spermidine is preferred over spermine | Danio rerio | CoA + N1-acetylspermine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Danio rerio |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
58.2 | - |
melting temperature | Danio rerio |
85 | - |
irreversible heat denaturing | Danio rerio |