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Literature summary for 2.3.1.57 extracted from

  • Niiyama, M.; Sugiyama, S.; Hirose, M.; Ishikawa, S.; Tomitori, H.; Higashi, K.; Yamashita, T.; Adachi, H.; Takano, K.; Murakami, S.; Murata, M.; Inoue, T.; Mori, Y.; Kashiwagi, K.; Matsumura, H.; Igarashi, K.
    Expression, purification, crystallization and preliminary crystallographic analysis of spermidine acetyltransferase from Escherichia coli (2013), Acta Crystallogr. Sect. F, 69, 884-887.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene speG, recombinant enzyme expression in Escherichia coli strain BL21(DE3) Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, sitting-drop vapour-diffusion method, mixing of 0.001 ml of 40 mg/ml protein in 10 mM HEPES-KOH pH 7.5, 50 mM CoA, 50 mM spermidine, 300 mM KCl, 0.1 mM EDTA, 6 mM 2-mercaptoethanol, 10% glycerol, 10 mM PMSF, 0.02% Brij-35, with 0.001 ml of reservoir solution containing 50 mM sodium cacodylate, pH 6.5, 9% w/v PEG 8000, 0.1 M calcium acetate, and equilibration against 0.5 ml reservoir solution, 20°C, a few days, X-ray diffraction structure determination and analysis at 2.5 A resolution Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
-
Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3 acetyl-CoA + spermidine Escherichia coli
-
3 CoA + N1,N4,N8-triacetylspermidine
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
gene speG
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography and concanavalin A affinity chromatography, followed by gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 acetyl-CoA + spermidine
-
Escherichia coli 3 CoA + N1,N4,N8-triacetylspermidine
-
?

Synonyms

Synonyms Comment Organism
SAT
-
Escherichia coli
spermidine acetyltransferase
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
Escherichia coli

General Information

General Information Comment Organism
physiological function a key enzyme that transfers the acetyl group from acetyl-CoA to either the N-1 or N-8 position of spermidine, thereby reducing the intracellular polyamine level Escherichia coli