Cloned (Comment) | Organism |
---|---|
gene speG, recombinant enzyme expression in Escherichia coli strain BL21(DE3) | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, sitting-drop vapour-diffusion method, mixing of 0.001 ml of 40 mg/ml protein in 10 mM HEPES-KOH pH 7.5, 50 mM CoA, 50 mM spermidine, 300 mM KCl, 0.1 mM EDTA, 6 mM 2-mercaptoethanol, 10% glycerol, 10 mM PMSF, 0.02% Brij-35, with 0.001 ml of reservoir solution containing 50 mM sodium cacodylate, pH 6.5, 9% w/v PEG 8000, 0.1 M calcium acetate, and equilibration against 0.5 ml reservoir solution, 20°C, a few days, X-ray diffraction structure determination and analysis at 2.5 A resolution | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
- |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 acetyl-CoA + spermidine | Escherichia coli | - |
3 CoA + N1,N4,N8-triacetylspermidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene speG | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography and concanavalin A affinity chromatography, followed by gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 acetyl-CoA + spermidine | - |
Escherichia coli | 3 CoA + N1,N4,N8-triacetylspermidine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SAT | - |
Escherichia coli |
spermidine acetyltransferase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | a key enzyme that transfers the acetyl group from acetyl-CoA to either the N-1 or N-8 position of spermidine, thereby reducing the intracellular polyamine level | Escherichia coli |