Cloned (Comment) | Organism |
---|---|
gene ssat1, sequence comparison, expression profile, and phylogenetic analysis of ssat-like genes, recombinant expression of N-terminally GST- or His6-tagged enzyme in Escherichia coli strain BL21, recombinant expression in HEK-293T cells | Homo sapiens |
genes ssat1a, ssat1b, and ssat1c, sequence comparisons, expression profiles and phylogenetic analysis of ssat-like genes, translational regulatory mechanism analysis, recombinant expression of N-terminally GST- or His6-tagged enzymes in Escherichia coli strain BL21, recombinant expression of N-terminally GST- or His6-tagged enzymes in HEK-293T cells | Danio rerio |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a chimeric gene named ssat1a248b, as it contains nucleotides 1-248 of ssat1a and the remainder of ssat1b. Chimeric genes ssat1a332b, ssat1a374b, ssat1a453b, ssat1b248a, ssat1b332a, ssat1b389a, and ssat1b467a are also prepared in this manner | Danio rerio |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.055 | - |
spermine | isozyme ssat1a, pH and temperature not specified in the publication | Danio rerio | |
0.09 | - |
spermidine | isozyme ssat1b, pH and temperature not specified in the publication | Danio rerio | |
0.091 | - |
spermine | isozyme ssat1b, pH and temperature not specified in the publication | Danio rerio | |
0.139 | - |
spermine | isozyme ssat1c, pH and temperature not specified in the publication | Danio rerio | |
0.182 | - |
spermidine | isozyme ssat1a, pH and temperature not specified in the publication | Danio rerio | |
0.232 | - |
spermidine | isozyme ssat1c, pH and temperature not specified in the publication | Danio rerio |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 acetyl-CoA + 2 spermine | Homo sapiens | - |
3 CoA + N1-acetylspermine + N1,N12-diacetylspermine | - |
? | |
3 acetyl-CoA + 2 spermine | Danio rerio | - |
3 CoA + N1-acetylspermine + N1,N12-diacetylspermine | - |
? | |
3 acetyl-CoA + spermidine | Homo sapiens | - |
3 CoA + N1,N4,N8-triacetylspermidine | - |
? | |
3 acetyl-CoA + spermidine | Danio rerio | - |
3 CoA + N1,N4,N8-triacetylspermidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | Q6GQM2 | Ssat1; three homologous ssat1 genes, named ssat1a, ssat1b, and ssat1c | - |
Homo sapiens | P21673 | Ssat1 | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally GST- or His6-tagged enzyme from Escherichia coli strain BL21 by affinity chromatography | Homo sapiens |
recombinant N-terminally GST- or His6-tagged enzymes from Escherichia coli strain BL21 by affinity chromatography | Danio rerio |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Danio rerio | - |
eye | - |
Danio rerio | - |
fin | - |
Danio rerio | - |
gill | - |
Danio rerio | - |
gut | - |
Danio rerio | - |
heart | - |
Danio rerio | - |
kidney | - |
Danio rerio | - |
liver | - |
Danio rerio | - |
additional information | genes ssat1a, ssat1b, and ssat1c ares ubiquitously expressed in fish tissues st different levels, expression patterns, overview | Danio rerio | - |
muscle | - |
Danio rerio | - |
ovary | - |
Danio rerio | - |
spleen | - |
Danio rerio | - |
testis | - |
Danio rerio | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 acetyl-CoA + 2 spermine | - |
Homo sapiens | 3 CoA + N1-acetylspermine + N1,N12-diacetylspermine | - |
? | |
3 acetyl-CoA + 2 spermine | - |
Danio rerio | 3 CoA + N1-acetylspermine + N1,N12-diacetylspermine | - |
? | |
3 acetyl-CoA + spermidine | - |
Homo sapiens | 3 CoA + N1,N4,N8-triacetylspermidine | - |
? | |
3 acetyl-CoA + spermidine | - |
Danio rerio | 3 CoA + N1,N4,N8-triacetylspermidine | - |
? | |
additional information | human SSAT1 binds to the PAS-B (Per-ARNT-Sim) domain of HIF-1alpha, a key regulator of oxygen homeostasis in all metazoans, facilitating its degradation | Homo sapiens | ? | - |
? | |
additional information | polyamine-dependent protein synthesis is only found in isozymes Ssat1b and Ssat1c, not in Ssat1a. Also only Ssat1b and Ssat1c, but not the polyamine-insensitive Ssat1a, are able to interact with integrin alpha9 and Hif-1alpha. Substrate preferences of the isoenzymes are different. Ssat1b has similar Km values for spermidine and spermine, while Ssat1a has a smaller Km toward spermidine and Ssat1c has a smaller Km for spermine. Ssat1a and Ssatb have a better kcat/Km value for spermidine than that for spermine | Danio rerio | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
spermidine/spermine N1-acetyltransferase 1 | - |
Homo sapiens |
spermidine/spermine N1-acetyltransferase 1 | - |
Danio rerio |
SSAT1 | - |
Homo sapiens |
SSAT1 | - |
Danio rerio |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Homo sapiens | |
acetyl-CoA | - |
Danio rerio |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic analysis of ssat-like genes dividing the genes into 3 clusters, comparison of zebrafish and human gene sequences and regulation, overview | Homo sapiens |
evolution | phylogenetic analysis of ssat-like genes dividing the genes into 3 clusters, comparison of zebrafish and human gene sequences and regulation, overview. Zebrafish ssat1 homologues are paralogous genes which experience subfunctionalization in their function and regulation | Danio rerio |
metabolism | spermidine/spermine N1-acetyltransferase 1 is a key enzyme in the polyamine interconversion pathway, which maintains polyamine homeostasis | Homo sapiens |
metabolism | spermidine/spermine N1-acetyltransferase 1 is a key enzyme in the polyamine interconversion pathway, which maintains polyamine homeostasis | Danio rerio |
physiological function | Ssat1b and Ssat1c might not only be a polyamine metabolic enzyme but also simultaneously respond to polyamine levels and engage in cross-talk with other signaling pathways. They interact with a mammalian specific integrin alpha9 and Hif-1alpha, a key regulator of oxygen homeostasis in all metazoans | Danio rerio |
physiological function | the enzyme maintains polyamine homeostasis, mammalian Ssat1 is also involved in many physiological and pathological events such as hypoxia, cell migration, and carcinogenesis | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
spermidine | isozyme ssat1c, pH and temperature not specified in the publication | Danio rerio | |
1.9 | - |
spermine | isozyme ssat1c, pH and temperature not specified in the publication | Danio rerio | |
4.3 | - |
spermine | isozyme ssat1b, pH and temperature not specified in the publication | Danio rerio | |
34.1 | - |
spermidine | isozyme ssat1b, pH and temperature not specified in the publication | Danio rerio | |
62.4 | - |
spermine | isozyme ssat1a, pH and temperature not specified in the publication | Danio rerio | |
122.2 | - |
spermidine | isozyme ssat1a, pH and temperature not specified in the publication | Danio rerio |