Protein Variants | Comment | Organism |
---|---|---|
R452A | kinetic as well chemical modification studies have implicated the presence of an active site arginine in enzyme, whose function is to stabilize coenzyme binding, conserved arginines are converted to identify these residues | Rattus norvegicus |
R453A | kinetic as well chemical modification studies have implicated the presence of an active site arginine in enzyme, whose function is to stabilize coenzyme binding, conserved arginines are converted to identify these residues | Rattus norvegicus |
R458A | kinetic as well chemical modification studies have implicated the presence of an active site arginine in enzyme, whose function is to stabilize coenzyme binding, conserved arginines are converted to identify these residues | Rattus norvegicus |
R463A | kinetic as well chemical modification studies have implicated the presence of an active site arginine in enzyme, whose function is to stabilize coenzyme binding, conserved arginines are converted to identify these residues | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00025 | - |
CoA | - |
Rattus norvegicus | |
0.0004 | - |
Acetylcholine | - |
Rattus norvegicus | |
0.0004 | - |
CoA | enzyme mutant R458A | Rattus norvegicus | |
0.001 | - |
CoA | enzyme mutant R453A | Rattus norvegicus | |
0.0011 | - |
Acetylcholine | enzyme mutant R463A | Rattus norvegicus | |
0.0015 | - |
Acetylcholine | enzyme mutant R453A | Rattus norvegicus | |
0.0016 | - |
CoA | enzyme mutant R463A | Rattus norvegicus | |
0.002 | - |
Acetylcholine | enzyme mutant R458A | Rattus norvegicus | |
0.0092 | - |
Acetylcholine | enzyme mutant R452A | Rattus norvegicus | |
0.0154 | - |
CoA | enzyme mutant R452A | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + choline | - |
Rattus norvegicus | acetylcholine + CoA | - |
? |