Cloned (Comment) | Organism |
---|---|
large-scale expression as His-tag or chitin-binding-domain fusion protein in Escherichia coli, His-tag fusion protein expresses in inclusion bodies at 37°C growth temperature and in soluble form at 20°C, chitin-binding-domain fusion protein expressed at 15°C but not at 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + choline | Homo sapiens | synthesis of the neurotransmitter acetylcholine, deficiency in ChAT involved in several neurological and psychiatric disorders | acetylcholine + CoA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Oxidation Stability | Organism |
---|---|
extremely sensitive to oxidation due to its cysteine-rich nature, oxidation causes formation of dimers and loss of activity | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant proteins using His-tag or chitin-binding-domain | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
placenta | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
50 | - |
purified enzyme, pH 7.4 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + choline | - |
Homo sapiens | acetylcholine + CoA | - |
r | |
acetyl-CoA + choline | synthesis of the neurotransmitter acetylcholine, deficiency in ChAT involved in several neurological and psychiatric disorders | Homo sapiens | acetylcholine + CoA | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | gel filtration, dimerization caused by oxidation can be reversed by addition of reducing agents | Homo sapiens |