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Literature summary for 2.3.1.65 extracted from
- The co-purification and common identity of cholyl CoA:glycine- and cholyl CoA: taurine-N-acyltransferase activities from bovine liver (1979), J. Biol. Chem., 254, 2059-2063.
General Stability
| General Stability | Organism |
|---|---|
| freezing inactivates | Bos taurus |
| high ionic strength stabilizes | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Bos taurus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Bos taurus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
gel filtration | Bos taurus |
| 50900 | - |
1 * 50900, SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| choloyl-CoA + glycine | Bos taurus | conjugation of bile acids in the liver with glycine or taurine prior to being secreted into the bile duct | CoA + glycocholate | - |
? |  |
| choloyl-CoA + taurine | Bos taurus | conjugation of bile acids in the liver with glycine or taurine prior to being secreted into the bile duct | CoA + taurocholate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 900fold | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 35.2 | - |
taurine | Bos taurus |
| 46.9 | - |
glycine | Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, 50 mM phosphate buffer, pH 7.8, with 0.5 M NaCl, 2 mM dithioerythritol, 1 month, 25% loss of activity | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| choloyl-CoA + glycine | - |
Bos taurus | CoA + glycocholate | - |
? | |
| choloyl-CoA + glycine | conjugation of bile acids in the liver with glycine or taurine prior to being secreted into the bile duct | Bos taurus | CoA + glycocholate | - |
? | |
| choloyl-CoA + taurine | - |
Bos taurus | CoA + taurocholate | - |
? | |
| choloyl-CoA + taurine | conjugation of bile acids in the liver with glycine or taurine prior to being secreted into the bile duct | Bos taurus | CoA + taurocholate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 50900, SDS-PAGE | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
taurocholate stabilizes against temperature denaturation | Bos taurus |
| 50 | - |
10 min, stable up to | Bos taurus |
| 57 | - |
10 min, about 70% loss of activity | Bos taurus |
| 60 | - |
inactivation within 10 min | Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | higher kcat for glycine than for taurine | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 6.6 | Bos taurus |
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Release 2023.1 (January 2023)
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