Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 2fold activation, lowers the Km-value | Homo sapiens | |
L-cysteine | 2fold activation | Homo sapiens | |
reduced glutathione | 2fold activation | Homo sapiens |
General Stability | Organism |
---|---|
glycerol, 40% v/v, stabilizes during storage | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chenodeoxycholate | - |
Homo sapiens | |
cholate | - |
Homo sapiens | |
cholesterol | - |
Homo sapiens | |
deoxycholate | - |
Homo sapiens | |
glycocholate | - |
Homo sapiens | |
glycodeoxycholate | - |
Homo sapiens | |
additional information | not inhibited by 2-mercaptoethanol, CaCl2, EDTA, glutathione | Homo sapiens | |
taurocholate | - |
Homo sapiens | |
taurodeoxycholate | - |
Homo sapiens | |
ursodeoxycholate | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
choloyl-CoA | + 60 mM glycine | Homo sapiens | |
0.087 | - |
choloyl-CoA | + 40 mM taurine | Homo sapiens | |
0.6 | - |
taurine | - |
Homo sapiens | |
3.2 | - |
glycine | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
2 * 52000, SDS-PAGE | Homo sapiens |
100000 | - |
gel filtration | Homo sapiens |
118000 | - |
sucrose density gradient centrifugation | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
choloyl-CoA + glycine | Homo sapiens | taurine can replace glycine, involved in conjugation of bile acids | CoA + glycocholate | - |
? | |
choloyl-CoA + glycine | Homo sapiens | cholesterol degradation, glycine conjugates of bile acids occur in adult | CoA + glycocholate | - |
? | |
choloyl-CoA + taurine | Homo sapiens | taurine can replace glycine, involved in conjugation of bile acids | CoA + taurocholate | - |
? | |
choloyl-CoA + taurine | Homo sapiens | cholesterol degradation, bile acids are secreted as taurine conjugates in fetus and neonatus | CoA + taurocholate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
about 100fold | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.42 | - |
taurine | Homo sapiens |
0.43 | - |
glycine | Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, in the presence of 40% v/v glycerol | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chenodeoxycholoyl-CoA + glycine | 91.4% of activity with choloyl-CoA | Homo sapiens | CoA + glycochenodeoxycholate | - |
? | |
chenodeoxycholoyl-CoA + taurine | 101.4% of activity with choloyl-CoA | Homo sapiens | CoA + taurochenodeoxycholate | - |
? | |
choloyl-CoA + beta-alanine | about 10% of the rate with glycine | Homo sapiens | CoA + beta-alanylcholate | - |
? | |
choloyl-CoA + D-alpha-alanine | about 10% of the rate with glycine | Homo sapiens | CoA + D-alpha-alanylcholate | - |
? | |
choloyl-CoA + glycine | ratio of glycine-/taurine-dependent activity is 1.0-1.4 | Homo sapiens | CoA + glycocholate | - |
? | |
choloyl-CoA + glycine | taurine can replace glycine, involved in conjugation of bile acids | Homo sapiens | CoA + glycocholate | - |
? | |
choloyl-CoA + glycine | cholesterol degradation, glycine conjugates of bile acids occur in adult | Homo sapiens | CoA + glycocholate | - |
? | |
choloyl-CoA + taurine | ratio of glycine-/taurine-dependent activity is 1.0-1.4 | Homo sapiens | CoA + taurocholate | - |
? | |
choloyl-CoA + taurine | taurine can replace glycine, involved in conjugation of bile acids | Homo sapiens | CoA + taurocholate | - |
? | |
choloyl-CoA + taurine | cholesterol degradation, bile acids are secreted as taurine conjugates in fetus and neonatus | Homo sapiens | CoA + taurocholate | - |
? | |
deoxycholoyl-CoA + glycine | 92.4% of activity with choloyl-CoA | Homo sapiens | CoA + glycodeoxycholate | - |
? | |
deoxycholoyl-CoA + taurine | 81.7% of activity with choloyl-CoA | Homo sapiens | CoA + taurodeoxycholate | - |
? | |
lithocholoyl-CoA + glycine | 58.4% of activity with choloyl-CoA | Homo sapiens | CoA + glycolithocholate | - |
? | |
lithocholoyl-CoA + taurine | 43% of activity with choloyl-CoA | Homo sapiens | CoA + taurolithocholate | - |
? | |
additional information | not: L-alpha-alanine, DL-cysteic acid, 4-aminobutyric acid, cysteine, sulfanilic acid | Homo sapiens | ? | - |
? | |
ursodeoxycholoyl-CoA + glycine | 24.8% of activity with choloyl-CoA | Homo sapiens | CoA + glycoursodeoxycholate | - |
? | |
ursodeoxycholoyl-CoA + taurine | 35.3% of activity with choloyl-CoA | Homo sapiens | CoA + tauroursodeoxycholate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 52000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 5.85 | Homo sapiens |
6.5 | - |
taurine-dependent activity | Homo sapiens |
7.2 | - |
glycine-dependent activity | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.6 | 8.2 | taurine: about half-maximal activity at pH 5.2 and pH 8.2, glycine: 80% of maximal activity at pH 8.2 | Homo sapiens |