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Literature summary for 2.3.1.67 extracted from

  • Gomez-Cambronero, J.; Velasco, S.; Sanchez-Crespo, M.; Vivanco, F.; Mato, J.M.
    Partial purification and characterization of 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine: acetyl-CoA acetyltransferase from rat spleen (1986), Biochem. J., 237, 439-445.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.67

General Stability

General Stability Organism
stable to lyophilization Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
sodium dodecylsulfate inactivation at 0.1% Rattus norvegicus
Urea inactivation at 8 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.137
-
 acetyl-CoA
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 x * 29000, SDS-PAGE and affinity labelling experiments Rattus norvegicus
800000
-
 MW after gel filtration, aggregate of several proteins or protein aggregates coated with lipids Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
solubilization in sodium deoxycholate, precipitation by ammonium sulfate, ultragel AcA-22 chromatography, DEAE-sepharose CL-6B chromatography, covalent chromatography with thiopropyl-sepharose 6B Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-
liver
-
 Canis lupus familiaris
-
spleen
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus
additional information
-
-
 Canis lupus familiaris
0.317
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 1-palmitoyl-2-lyso-sn-glycero-3-phosphocholine 12% of the activity observed in presence of alkyl-lyso-sn-glycero-phosphocholine Rattus norvegicus CoA + 2-acetyl-1-palmitoyl-sn-glycero-3-phosphocholine
-
 ?
acetyl-CoA + alkyl-sn-glycero-3-phosphocholine
-
 Rattus norvegicus CoA + 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine
-
 ?
oleoyl-CoA + alkyl-lyso-glycero-3-phosphoethanolamine 10% of the activity observed in presence of acetyl-CoA Rattus norvegicus CoA + 1-alkyl-2-oleoyl-glycero-3-phosphoethanolamine
-
 ?
palmitoyl-CoA + alkyl-lyso-glycero-3-phosphoethanolamine 5% of the activity observed in presence of acetyl-CoA Rattus norvegicus CoA + acetyl-2-palmitoyl-glycero-3-phosphoethanolamine
-
 ?

Subunits

Subunits Comment Organism
? x * 29000, SDS-PAGE and affinity labelling experiments Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Rattus norvegicus