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Literature summary for 2.3.1.75 extracted from
- Purification of a jojoba embryo wax synthase, cloning of its cDNA, and production of high levels of wax in seeds of transgenic Arabidopsis (2000), Plant Physiol., 122, 645-655.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | transgenic plants expressing the enzyme allow the production of long-chain liquid waxes at reasonable cost for use in commercial applications | Simmondsia chinensis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning of cDNA, DNA sequence analysis, construction of plasmid to express the cDNA in transgenic Arabidopsis thaliana plants | Simmondsia chinensis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | cloned cDNA is expressed in transgenic Arabidopsis thaliana plants, in combination with cDNAs encoding the jojoba fatty acyl-CoA reductase and a beta-ketoacyl-CoA synthase from Lunaria annua under a seed specific promotor, highly enhanced wax content in transgenic seeds | Simmondsia chinensis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Simmondsia chinensis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid sequence analysis and alignment | Simmondsia chinensis |
| 30000 | - |
x * 30000, SDS-PAGE | Simmondsia chinensis |
| 40000 | - |
gel filtration | Simmondsia chinensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Simmondsia chinensis | Q9XGY6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially | Simmondsia chinensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | developing | Simmondsia chinensis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| eicosenoyl-CoA + octadecenol | best substrates | Simmondsia chinensis | octadecenyl eicosenoate + CoA | - |
? |  |
| additional information | substrate specificity, overview | Simmondsia chinensis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, SDS-PAGE | Simmondsia chinensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| fatty acyl-coenzyme A:fatty alcohol acyltransferase | - |
Simmondsia chinensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 9.8 | Simmondsia chinensis |
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Release 2023.1 (January 2023)
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