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Literature summary for 2.3.1.75 extracted from
- Both histidine residues of the conserved HHXXXDG motif are essential for wax ester synthase/acyl-CoA:diacylglycerol acyltransferase catalysis (2009), Eur. J. Lipid Sci. Technol., 111, 112-119.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene atfA, expression of wild-type and mutant enzymes in Escherichia coli strain Rosetta(DE3) | Acinetobacter baylyi |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D137A | the mutant shows slightly reduced activity compared to the wild-type enzyme | Acinetobacter baylyi |
| G138A | the mutant shows slightly reduced activity compared to the wild-type enzyme | Acinetobacter baylyi |
| H132L | the replacement of His132 leads to reduced enzyme activity | Acinetobacter baylyi |
| H132L/H133L | inactive mutant | Acinetobacter baylyi |
| H133L | the replacement of His133 leads to highly reduced enzyme activity | Acinetobacter baylyi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter baylyi | - |
gene atfA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant enzymes from Escherichia coli by cation exchange and hydrophobic interaction chromatography, followed by anion exchange chromatography | Acinetobacter baylyi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00000005 | - |
recombinant mutant H133L | Acinetobacter baylyi |
| 0.0000005 | - |
recombinant mutant H132L | Acinetobacter baylyi |
| 0.0000414 | - |
recombinant mutant D137A | Acinetobacter baylyi |
| 0.0000464 | - |
recombinant wild-type enzyme | Acinetobacter baylyi |
| 0.0000517 | - |
recombinant mutant G138A | Acinetobacter baylyi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | WS/DGAT is a very unspecific enzyme and accepts a wide variety of hydrophobic substrates, although also several compounds, e.g. hydroxylamine, sugars, and astaxanthine, do not serve as substrates, substrate specificity, overview. The highly conserved HHXXXDG motif is located at the enzyme's active site, it possesses two histidine residues that are essential for catalysis | Acinetobacter baylyi | ? | - |
? |  |
| palmitoyl-CoA + 1,2-dipalmitin | - |
Acinetobacter baylyi | ? | - |
? | |
| palmitoyl-CoA + 1-hexadecanol | - |
Acinetobacter baylyi | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATF | - |
Acinetobacter baylyi |
| wax ester synthase/acyl-CoA:diacylglycerol acyltransferase | - |
Acinetobacter baylyi |
| WS/DGAT | - |
Acinetobacter baylyi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
assay at | Acinetobacter baylyi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Acinetobacter baylyi |
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Release 2023.1 (January 2023)
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