Literature summary for 2.3.1.76 extracted from

Orland, M.D.; Anwar, K.; Cromley, D.; Chu, C.; Chen, L.; Billheimer, J.T.; Hussain, M.M.; Cheng, D.
Acyl coenzyme A dependent retinol esterification by acyl coenzyme A:diacylglycerol acyltransferase 1 (2005), Biochim. Biophys. Acta, 1737, 76-82.

Search for more literature for 2.3.1.76

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Spodoptera frugiperda Sf9 cell membranes using the baculovrisu transfection system	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
XP620	inhibits the ARAT activity of acyl coenzyme A:diacylglycerol acyltransferase isozyme 1 dependent on the retinol concentration in vivo and in vitro, IC50: 110 nM, no inhibition of isozyme 2 and acyl coenzyme A:monoacylglycerol acyltransferase isozyme 3, overview	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0139	-	palmitoyl-CoA	ARAT activity of recombinant acyl coenzyme A:diacylglycerol acyltransferase isozyme 1, pH 7.4, 37°C	Homo sapiens
0.0259	-	retinol	ARAT activity of recombinant acyl coenzyme A:diacylglycerol acyltransferase isozyme 1, pH 7.4, 37°C	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Homo sapiens	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acyl-CoA + retinol	Homo sapiens	-	CoA + retinyl ester	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
Caco-2 cell	colon cancer cell line	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.00045	-	ARAT activity of recombinant acyl coenzyme A:diacylglycerol acyltransferase isozyme 2 in SF9 cell membrane preparation	Homo sapiens
0.00084	-	ARAT activity of recombinant acyl coenzyme A:monoacylglycerol acyltransferase isozyme 3 in SF9 cell membrane preparation	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acyl-CoA + retinol	-	Homo sapiens	CoA + retinyl ester	-	?
additional information	acyl coenzyme A:diacylglycerol acyltransferase, DGAT, EC 2.3.1.20, and acyl coenzyme A:monoacylglycerolol acyltransferase, MGAT, EC 2.3.1.22, also show activity with retinol, overview	Homo sapiens	?	-	?
palmitoyl-CoA + retinol	-	Homo sapiens	CoA + retinyl palmitate	-	?

Synonyms

Synonyms	Comment	Organism
acyl coenzyme A:retinol acyltransferase	-	Homo sapiens
ArAT	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00045	-	XP620	ARAT activity of acyl coenzyme A:diacylglycerol acyltransferase isozyme 1, pH 7.4, 37°C	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00011	-	inhibits the ARAT activity of acyl coenzyme A:diacylglycerol acyltransferase isozyme 1 dependent on the retinol concentration in vivo and in vitro, IC50: 110 nM, no inhibition of isozyme 2 and acyl coenzyme A:monoacylglycerol acyltransferase isozyme 3, ov	Homo sapiens	XP620

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Release 2023.1 (January 2023)