Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Rattus norvegicus | |
coenzyme A | - |
Rattus norvegicus | |
additional information | iodoacetamide and N-ethylmaleimide are not inhibitory | Rattus norvegicus | |
N-acetyl-alpha-D-glucosamine | - |
Rattus norvegicus | |
N-acetyl-beta-D-glucosamine | - |
Rattus norvegicus | |
p-chloromercuribenzoate | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | 0.3 | alpha-D-glucosamine | - |
Rattus norvegicus | |
0.55 | - |
acetyl-CoA | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | enzyme is acetylated at the cytoplasmic side of the lysosome and the acetyl group is then transferred to the inside where it is used to acetylate heparan sulfate | Rattus norvegicus | 5764 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + heparan sulfate alpha-D-glucosaminide | Rattus norvegicus | - |
CoA + heparan sulfate N-acetyl-alpha-D-glucosaminide | - |
? | |
additional information | Rattus norvegicus | enzyme is acetylated at the cytoplasmic side of the lysosome and the acetyl group is then transferred to the inside where it is used to acetylate heparan sulfate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + heparan sulfate alpha-D-glucosaminide = CoA + heparan sulfate N-acetyl-alpha-D-glucosaminide | di-iso ping-pong mechanism | Rattus norvegicus | |
acetyl-CoA + heparan sulfate alpha-D-glucosaminide = CoA + heparan sulfate N-acetyl-alpha-D-glucosaminide | transmembranal reaction mechanism | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + alpha-D-glucosamine | - |
Rattus norvegicus | CoA + N-acetyl-alpha-D-glucosamine | - |
? | |
acetyl-CoA + heparan sulfate alpha-D-glucosaminide | - |
Rattus norvegicus | CoA + heparan sulfate N-acetyl-alpha-D-glucosaminide | - |
? | |
acetyl-CoA + heparan sulfate alpha-D-glucosaminide | brings about the acetylation of glucosamine groups of heparan sulfate and heparin from which the sulfate has been removed | Rattus norvegicus | CoA + heparan sulfate N-acetyl-alpha-D-glucosaminide | - |
? | |
additional information | enzyme is acetylated at the cytoplasmic side of the lysosome and the acetyl group is then transferred to the inside where it is used to acetylate heparan sulfate | Rattus norvegicus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
transmembrane transfer | Rattus norvegicus |
6 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 5.5 | pH 5.0: about 30% of activity maximum, pH 5.5: maximum activity above | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
coenzyme A | versus acetyl-CoA | Rattus norvegicus | |
7 | - |
coenzyme A | versus N-acetyl-alpha-D-glucosamine | Rattus norvegicus | |
15 | - |
N-acetyl-alpha-D-glucosamine | versus glusosamine, no detergents added | Rattus norvegicus | |
16.5 | - |
N-acetyl-alpha-D-glucosamine | versus acetyl-CoA, no detergents added | Rattus norvegicus |