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Literature summary for 2.3.1.8 extracted from
- Phosphotransacetylase aus Clostridium kluyveri (1963), Biochem. Z., 338, 114-121.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Clostridium kluyveri |
General Stability
| General Stability | Organism |
|---|---|
| dilute solutions are instable, in frozen state stable | Clostridium kluyveri |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-dipyridyl | - |
Clostridium kluyveri |  |
| CuSO4 | - |
Clostridium kluyveri | |
| K+ | - |
Clostridium kluyveri | |
| KCN | weak | Clostridium kluyveri | |
| Mg2+ | - |
Clostridium kluyveri | |
| MnCl2 | weak | Clostridium kluyveri | |
| Na+ | - |
Clostridium kluyveri | |
| p-chloromercuribenzoate | - |
Clostridium kluyveri | |
| Tris(hydroxymethyl)aminomethane | weak | Clostridium kluyveri | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.56 | - |
CoA | - |
Clostridium kluyveri | |
| 0.66 | - |
acetyl phosphate | - |
Clostridium kluyveri | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NH4+ | required for maximum activity, optimum at 7 mM (NH4)2SO4 | Clostridium kluyveri | |
| phosphate | 1 mM, weak activation | Clostridium kluyveri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | 41000 | ultracentrifugation | Clostridium kluyveri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + phosphate | Clostridium kluyveri | - |
CoA + acetyl phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium kluyveri | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clostridium kluyveri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9100 | - |
after crystallization | Clostridium kluyveri |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, 2.7-3.0 M ammonium sulfate, months | Clostridium kluyveri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + phosphate | - |
Clostridium kluyveri | CoA + acetyl phosphate | - |
r | |
| acetyl-CoA + phosphate | in reverse reaction specific for CoA | Clostridium kluyveri | CoA + acetyl phosphate | - |
r | |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | 40 | only small effect of temperature in this range | Clostridium kluyveri |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
5 min, 1.5 mg/ml protein concentration, 0.15 M ammonium sulfate, pH 6, stable | Clostridium kluyveri |
| 45 | - |
5 min, 1.5 mg/ml protein concentration, 0.15 M ammonium sulfate, pH 6, 15% loss of activity | Clostridium kluyveri |
| 50 | - |
5 min, 1.5 mg/ml protein concentration, 0.15 M ammonium sulfate, pH 6, 48% loss of activity | Clostridium kluyveri |
| 60 | - |
5 min, 1.5 mg/ml protein concentration, 0.15 M ammonium sulfate, pH 6, 98% loss of activity | Clostridium kluyveri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Clostridium kluyveri |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | 8.2 | about 50% of activity maximum at pH 6.6 pH and 8.2 | Clostridium kluyveri |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
1.5 mg/ml protein concentration, 0.15 M ammonium sulfate, 40°C, 5 min, stable | Clostridium kluyveri |
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