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Literature summary for 2.3.1.8 extracted from

  • Robinson, J.R.; Sagers, R.D.
    Phosphotransacetylase from Clostridium acidiurici (1972), J. Bacteriol., 112, 465-473.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.8

General Stability

General Stability Organism
50-60% loss of activity after dialysis for 8 h against 0.05 M Tris buffer, Fe2+ and dithiothreitol stabilize Gottschalkia acidurici

Inhibitors

Inhibitors Comment Organism Structure
arsenate 50% inhibition at 10 mM Gottschalkia acidurici
Diethylbarbiturate
-
 Gottschalkia acidurici
diphosphate 50% inhibition at 12 mM Gottschalkia acidurici
additional information inhibition by various buffer systems Gottschalkia acidurici
phosphate
-
 Gottschalkia acidurici
Tris-citrate
-
 Gottschalkia acidurici

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required, Fe2+ or Mn2+, Mn2+ is 50-75% as effective as Fe2+ Gottschalkia acidurici
Mn2+ required, Fe2+ or Mn2+, Mn2+ is 50-75% as effective as Fe2+ Gottschalkia acidurici
additional information no activity with: Fe3+, Zn2+, Mg2+, Co2+, Ni2+, Sn2+, Cu2+, Mo6+, K+, NH4+ Gottschalkia acidurici

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000 75000 gel filtration Gottschalkia acidurici

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + phosphate Gottschalkia acidurici
-
 CoA + acetyl phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Gottschalkia acidurici
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gottschalkia acidurici

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7100
-
-
 Gottschalkia acidurici

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + phosphate
-
 Gottschalkia acidurici CoA + acetyl phosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
88
-
 60 min, 44% loss of activity Gottschalkia acidurici

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.1
-
 Gottschalkia acidurici

pH Range

pH Minimum pH Maximum Comment Organism
7 8.7 pH 7.0: about 10% of activity maximum, pH 8.7: about 20% of activity maximum Gottschalkia acidurici