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Literature summary for 2.3.1.8 extracted from
- Purification and properties of phosphotransacetylase from Lactobacillus fermenti (1971), J. Biochem., 69, 789-801.
General Stability
| General Stability | Organism |
|---|---|
| lyophilization causes almost complete loss of enzyme activity | Limosilactobacillus fermentum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | - |
Limosilactobacillus fermentum |  |
| Ca2+ | - |
Limosilactobacillus fermentum | |
| Cs+ | - |
Limosilactobacillus fermentum | |
| Li+ | - |
Limosilactobacillus fermentum | |
| Mg2+ | - |
Limosilactobacillus fermentum | |
| N-ethylmaleimide | above 0.1 mM | Limosilactobacillus fermentum | |
| p-chloromercuribenzoate | above 0.1 mM | Limosilactobacillus fermentum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.087 | - |
CoA | - |
Limosilactobacillus fermentum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | activates | Limosilactobacillus fermentum | |
| NH4+ | activates | Limosilactobacillus fermentum | |
| Rb+ | stimulates | Limosilactobacillus fermentum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
gel filtration | Limosilactobacillus fermentum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + phosphate | Limosilactobacillus fermentum | - |
CoA + acetyl phosphate | - |
r | |
| acetyl-CoA + phosphate | Limosilactobacillus fermentum DSM 20391 | - |
CoA + acetyl phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Limosilactobacillus fermentum | - |
IFO 3071 | - |
| Limosilactobacillus fermentum DSM 20391 | - |
IFO 3071 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Limosilactobacillus fermentum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1124 | - |
- |
Limosilactobacillus fermentum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-dephospho-CoA + acetyl phosphate | rate is about one tenth of the activity with CoA | Limosilactobacillus fermentum | acetyl-3'-dephospho-CoA + phosphate | - |
? | |
| 3'-dephospho-CoA + acetyl phosphate | rate is about one tenth of the activity with CoA | Limosilactobacillus fermentum DSM 20391 | acetyl-3'-dephospho-CoA + phosphate | - |
? | |
| acetyl-CoA + phosphate | - |
Limosilactobacillus fermentum | CoA + acetyl phosphate | - |
r | |
| acetyl-CoA + phosphate | - |
Limosilactobacillus fermentum DSM 20391 | CoA + acetyl phosphate | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Limosilactobacillus fermentum |
| 45 | - |
unstable, can be stabilized by ammonium sulfate | Limosilactobacillus fermentum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
Tris buffer | Limosilactobacillus fermentum |
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