Protein Variants | Comment | Organism |
---|---|---|
R133A | altered kinetic properties, increased Km for CoA | Methanosarcina thermophila |
R133E | altered kinetic properties, increased Km for CoA | Methanosarcina thermophila |
R133K | altered kinetic properties, increased Km for CoA | Methanosarcina thermophila |
R133Q | altered kinetic properties, increased Km for CoA | Methanosarcina thermophila |
R87A | altered kinetic properties, increased Km for CoA | Methanosarcina thermophila |
R87E | altered kinetic properties, increased Km for CoA | Methanosarcina thermophila |
R87K | altered kinetic properties, increased Km for CoA | Methanosarcina thermophila |
R87Q | altered kinetic properties, increased Km for CoA | Methanosarcina thermophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-Butanedione | almost complete loss of wild-type enzyme activity after 10 min at 10 mM | Methanosarcina thermophila | |
desulfo-CoA | strong competitive inhibitor | Methanosarcina thermophila | |
phosphate | - |
Methanosarcina thermophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
CoA | wild-type | Methanosarcina thermophila | |
0.11 | - |
CoA | R133K mutant | Methanosarcina thermophila | |
0.27 | - |
CoA | R87E mutant | Methanosarcina thermophila | |
0.36 | - |
CoA | R133A mutant | Methanosarcina thermophila | |
0.7 | - |
CoA | R133Q mutant | Methanosarcina thermophila | |
0.7 | - |
CoA | R133E mutant | Methanosarcina thermophila | |
0.75 | - |
CoA | R87K mutant | Methanosarcina thermophila | |
0.8 | - |
desulfo-CoA | R87E mutant | Methanosarcina thermophila | |
0.8 | - |
desulfo-CoA | R133K mutant | Methanosarcina thermophila | |
0.9 | - |
CoA | R87A mutant | Methanosarcina thermophila | |
1 | - |
desulfo-CoA | R133E mutant | Methanosarcina thermophila | |
1.1 | - |
CoA | R87Q mutant | Methanosarcina thermophila | |
1.3 | - |
desulfo-CoA | R87Q mutant | Methanosarcina thermophila | |
1.3 | - |
desulfo-CoA | R133A mutant | Methanosarcina thermophila | |
1.4 | - |
desulfo-CoA | wild-type | Methanosarcina thermophila | |
3.9 | - |
desulfo-CoA | R87K mutant | Methanosarcina thermophila | |
4 | - |
desulfo-CoA | R133Q mutant | Methanosarcina thermophila | |
6 | - |
desulfo-CoA | R87A mutant | Methanosarcina thermophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + phosphate | Methanosarcina thermophila | - |
CoA + acetyl phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina thermophila | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + phosphate | - |
Methanosarcina thermophila | CoA + acetyl phosphate | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00467 | - |
desulfo-CoA | R133E mutant | Methanosarcina thermophila | |
0.0125 | - |
desulfo-CoA | R133A mutant | Methanosarcina thermophila | |
0.133 | - |
desulfo-CoA | R133K mutant | Methanosarcina thermophila | |
0.283 | - |
CoA | R87E mutant | Methanosarcina thermophila | |
0.383 | - |
desulfo-CoA | R87E mutant | Methanosarcina thermophila | |
0.467 | - |
desulfo-CoA | R133Q mutant | Methanosarcina thermophila | |
0.683 | - |
desulfo-CoA | R87K mutant | Methanosarcina thermophila | |
0.733 | - |
CoA | R133E mutant | Methanosarcina thermophila | |
1.63 | - |
desulfo-CoA | R87Q mutant | Methanosarcina thermophila | |
3.07 | - |
desulfo-CoA | R87A mutant | Methanosarcina thermophila | |
4.53 | - |
desulfo-CoA | wild-type | Methanosarcina thermophila | |
6.22 | - |
CoA | R87K mutant | Methanosarcina thermophila | |
6.97 | - |
CoA | R133K mutant | Methanosarcina thermophila | |
10.5 | - |
CoA | R133A mutant | Methanosarcina thermophila | |
19.1 | - |
CoA | R133Q mutant | Methanosarcina thermophila | |
20.4 | - |
CoA | R87A mutant | Methanosarcina thermophila | |
23.2 | - |
CoA | R87Q mutant | Methanosarcina thermophila | |
81.3 | - |
CoA | wild-type | Methanosarcina thermophila |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
desulfo-CoA | - |
Methanosarcina thermophila | |
0.32 | - |
phosphate | R133Q mutant | Methanosarcina thermophila | |
0.52 | - |
phosphate | R87Q mutant | Methanosarcina thermophila | |
0.81 | - |
phosphate | wild-type enzyme | Methanosarcina thermophila |