Literature summary for 2.3.1.8 extracted from

Iyer, P.P.; Lawrence, S.H.; Yennawar, H.P.; Ferry, J.G.
Expression, purification, crystallization and preliminary X-ray analysis of phosphotransacetylase from Methanosarcina thermophila (2003), Acta Crystallogr. Sect. D, 59, 1517-1520.

Search for more literature for 2.3.1.8

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in soluble form in Escherichia coli BL21-DE3	Methanosarcina thermophila

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method	Methanosarcina thermophila

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
71300	-	calculated from hydrodynamic radius obtained from dynamic light scattering	Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acetyl-CoA + phosphate	Methanosarcina thermophila	-	CoA + acetyl phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Methanosarcina thermophila	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli	Methanosarcina thermophila

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + phosphate	-	Methanosarcina thermophila	CoA + acetyl phosphate	-	r

Subunits

Subunits	Comment	Organism
dimer	in solution, dynamic light scattering	Methanosarcina thermophila

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4500	-	acetyl-CoA	-	Methanosarcina thermophila

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Release 2023.1 (January 2023)