Cloned (Comment) | Organism |
---|---|
Pseudomonas aeruginosa plasmid pPK237 transferred to and expressed in Escherichia coli K12 | Pseudomonas aeruginosa |
General Stability | Organism |
---|---|
dialysis against EDTA with subsequent Mg2+-addition to dialyzed enzyme has no effect on activity | Pseudomonas aeruginosa |
gel filtration leads to complete inactivation | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | above 0.5 mM | Pseudomonas aeruginosa | |
additional information | no inhibition by NH4+, K+, Na+, DTT, N-methylmaleimide | Pseudomonas aeruginosa | |
Zn2+ | - |
Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.066 | - |
gentamicin C1 | - |
Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1.0 mM, about 100% activity enhancement | Pseudomonas aeruginosa | |
Mg2+ | 1.5 mM, about 100% activity enhancement | Pseudomonas aeruginosa | |
NH4+ | slight activation | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid analysis | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
plasmid pPK237 transferred to and expressed in Escherichia coli K12 | - |
Purification (Comment) | Organism |
---|---|
plasmid pPK237 transferred to and expressed in E. coli K12, affinity chromatography | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
34 | - |
- |
Pseudomonas aeruginosa |
Storage Stability | Organism |
---|---|
-20°C, partially purified, several months | Pseudomonas aeruginosa |
-20°C, purified, several days | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + dibekacin | acetylation at 8% the rate of gentamicin C1 | Pseudomonas aeruginosa | CoA + N3''-acetyldibekacin | - |
? | |
acetyl-CoA + gentamicin A | acetylation at 6% the rate of gentamicin C1 | Pseudomonas aeruginosa | CoA + N3'-acetylgentamicin A | - |
? | |
acetyl-CoA + gentamicin C | acetylation at 86% the rate of gentamicin C1 | Pseudomonas aeruginosa | CoA + N3'-acetylgentamicin C | - |
? | |
acetyl-CoA + gentamicin C1 | best substrate | Pseudomonas aeruginosa | CoA + N3'-acetylgentamicin C1 | - |
? | |
acetyl-CoA + gentamicin C1a | acetylation at 60% the rate of gentamicin C1 | Pseudomonas aeruginosa | CoA + N3'-acetylgentamicin C1a | - |
? | |
acetyl-CoA + gentamicin C2 | acetylation at 84% the rate of gentamicin C1 | Pseudomonas aeruginosa | CoA + N3'-acetylgentamicin C2 | - |
? | |
acetyl-CoA + kanamycin A | acetylation at 7% the rate of gentamicin C1 | Pseudomonas aeruginosa | CoA + N3'-acetylkanamycin A | - |
? | |
acetyl-CoA + sisomicin | acetylation at 51% the rate of gentamicin C1 | Pseudomonas aeruginosa | CoA + N3'-acetylsisomicin | - |
? | |
acetyl-CoA + tobramycin | acetylation at 6% the rate of gentamicin C1 | Pseudomonas aeruginosa | CoA + N3'-acetyltobramycin | - |
? | |
additional information | no substrates are amikacin | Pseudomonas aeruginosa | ? | - |
? | |
additional information | lividomycin A, butirosin A and B | Pseudomonas aeruginosa | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
above, inactivation within 2 min | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.6 | - |
Pseudomonas aeruginosa |